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NGFR/p75 neurotrophin receptor (p75NTR), Mouse Monoclonal Antibody (Saporin)
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Catalog Number
M-1755-IT
- Product Name NGFR/p75 neurotrophin receptor (p75NTR), Mouse Monoclonal Antibody (Saporin)
- Product Description MC192-saporin is an antibody conjugate comprising of the monoclonal antibody MC192 against rat p75NTR, the nerve growth factor receptor, chemically conjugated via a reducible disulfide bridge to the ribosome-inactivating protein saporin, purified from saponaria officinalis. Unconjugated saporin is incapable of entering the cells due to the apparent lack of ligand. Upon specific binding via MC192 to the cells expressing p75NTR, saporin transverses the cell membrane leading to lesion of neurochemically defined neuronal populations. The targets of MC192-Saporin are p75NTR-expressing cells including cholinergic neurons of the basal forebrain, cerebellar Purkinje cells, medial septum, diagonal band of Broca, Nucleus basalis of Meynert and some tumour cells. MC192-saporin has been used in the study of learning and memory and its primary application is in vivo, MC192-saporin is specific for applications in rat. The antibody does not cross-react with human or mouse p75NTR receptors.
- Alternative Names Low-affinity nerve growth factor receptor; NGF receptor; Gp80-LNGFR; p75 ICD; Low affinity neurotrophin receptor p75NTR; CD271
- Application(s) In-vivo
- Antibody Host Mouse
- Antibody Type Monoclonal
- Specificity MC192 antibody is specific only for rat NGFR, no reactivity to human or mouse NGFR has been reported This monoclonal antibody does not cross react with p75NTR-expressing cells in other species than rat.
- Species Reactivity Rat
- Immunogen Description Rat NGF receptor (p75NTR)
- Conjugate Saporin
- Purity Description Conjugate was purified by ion-exchange chromatography. Purity > 90% by non-reducing SDS-PAGE
- Regulatory Status For research use only.
Product Info
- Product Description MC192-saporin is an antibody conjugate comprising of the monoclonal antibody MC192 against rat p75NTR, the nerve growth factor receptor, chemically conjugated via a reducible disulfide bridge to the ribosome-inactivating protein saporin, purified from saponaria officinalis. Unconjugated saporin is incapable of entering the cells due to the apparent lack of ligand. Upon specific binding via MC192 to the cells expressing p75NTR, saporin transverses the cell membrane leading to lesion of neurochemically defined neuronal populations. The targets of MC192-Saporin are p75NTR-expressing cells including cholinergic neurons of the basal forebrain, cerebellar Purkinje cells, medial septum, diagonal band of Broca, Nucleus basalis of Meynert and some tumour cells. MC192-saporin has been used in the study of learning and memory and its primary application is in vivo, MC192-saporin is specific for applications in rat. The antibody does not cross-react with human or mouse p75NTR receptors.
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- Application(s) In-vivo
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Application Details
1. To specifically target and eliminate rat cells expressing p75NTR in vivo. MC192-saporin has been used to selectively lesion cholinergic neurons of basal forebrain to create an animal model to study Alzheimer's disease.
2. To be used as a model for gene delivery into neurons.
Biosensis recommends optimal dilutions/concentrations should be determined by the end user. - Target NGFR/p75 neurotrophin receptor (p75NTR)
- Specificity MC192 antibody is specific only for rat NGFR, no reactivity to human or mouse NGFR has been reported This monoclonal antibody does not cross react with p75NTR-expressing cells in other species than rat.
- Target Host Species Rat
- Species Reactivity Rat
- Antibody Host Mouse
- Antibody Type Monoclonal
- Antibody Isotype IgG1
- Clone Name MC192
- Conjugate Saporin
- Immunogen Description Rat NGF receptor (p75NTR)
- Biological Activity Active. Ablates p75-positive cells in rat in vivo. Routinely tested for dose-dependent killing of rat C6 cells in vitro. Note that the primary use of MC192-saporin is for in vivo applications in rat. Effective MC192-saporin concentrations must be determined for every new batch.
- Purity Description Conjugate was purified by ion-exchange chromatography. Purity > 90% by non-reducing SDS-PAGE
- Format Lyophilized from a 1 mg/mL solution containing PBS pH 7.2-7.6 without preservative.
- Reconstitution Instructions Spin vial briefly before opening. Reconstitute in sterile-filtered, ultrapure water to achieve a conjugate concentration of 1 mg/mL. Centrifuge to remove any insoluble material. Final buffer contains no preservatives.
- Storage Instructions Lyophilized product is shipped at ambient room temperature. Upon receipt, pulse-centrifuge the vial to collect solid that may be entrapped in the lid. After reconstitution, immediately prepare aliquots and keep the undiluted stock at -80°C for long-term storage. Avoid repeated thaw-freezing. For short-term storage, keep at 2-8°C for up to 2 weeks. it is recommended to handle this product under sterile conditions.
- Storage Temperature 2-8°C (lyophilized); <-70°C (liquid)
- Batch Number Please see item label.
- Expiration Date 12 months after date of receipt (unopened vial).
- Alternative Names Low-affinity nerve growth factor receptor; NGF receptor; Gp80-LNGFR; p75 ICD; Low affinity neurotrophin receptor p75NTR; CD271
- Uniprot Number P07174; P20656
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Uniprot Number/Name
P07174 (TNR16_RAT)
P20656 (RIP6_SAPOF) - Hazardous Statement Only qualified personnel should handle this product. MC192 antibody does not bind to human p75NTR receptor, however, standard laboratory protective measures should be followed, including wearing lab coat, gloves and safety glasses. Avoid contact with open wounds and practice good laboratory technique to avoid auto-exposure/auto-injection! Avoid contact of conjugate with reducing agents (eg., DTT, beta-mercaptoethanol) as this will destroy the antibody-saporin conjugate.
- Shipping Temperature 25°C (ambient)
- UNSPSC CODE 41116161
- Regulatory Status For research use only.
Specifications
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General References
Lehmann, O. et al (2003) Eur J Neurosci 18, 651-666
Power, A. E. et al (2002) Proc Natl Acad Sci U S A 99, 2315-2319
Perry, T. et al (2001) Brain Res Bull 54, 29-48
Wirth, S. et al (2000) Neuroreport 11, 347-350
5. Sherren, N. et al (1999) Brain Res Dev Brain Res 114, 49-62