Product DescriptiongoogleMC192-saporin is an antibody conjugate comprising of the monoclonal antibody MC192 against rat p75NTR, the nerve growth factor receptor, chemically conjugated via a reducible disulfide bridge to the ribosome-inactivating protein saporin, purified from saponaria officinalis. Unconjugated saporin is incapable of entering the cells due to the apparent lack of ligand. Upon specific binding via MC192 to the cells expressing p75NTR, saporin transverses the cell membrane leading to lesion of neurochemically defined neuronal populations. The targets of MC192-Saporin are p75NTR-expressing cells including cholinergic neurons of the basal forebrain, cerebellar Purkinje cells, medial septum, diagonal band of Broca, Nucleus basalis of Meynert and some tumour cells. MC192-saporin has been used in the study of learning and memory and its primary application is in vivo, MC192-saporin is specific for applications in rat. The antibody does not cross-react with human or mouse p75NTR receptors.
SpecificityMC192 antibody is specific only for rat NGFR, no reactivity to human or mouse NGFR has been reported This monoclonal antibody does not cross react with p75NTR-expressing cells in other species than rat.
Species ReactivityRat
Immunogen DescriptionRat NGF receptor (p75NTR)
ConjugateSaporin
Purity DescriptionConjugate was purified by ion-exchange chromatography. Purity > 90% by non-reducing SDS-PAGE
Product DescriptionMC192-saporin is an antibody conjugate comprising of the monoclonal antibody MC192 against rat p75NTR, the nerve growth factor receptor, chemically conjugated via a reducible disulfide bridge to the ribosome-inactivating protein saporin, purified from saponaria officinalis. Unconjugated saporin is incapable of entering the cells due to the apparent lack of ligand. Upon specific binding via MC192 to the cells expressing p75NTR, saporin transverses the cell membrane leading to lesion of neurochemically defined neuronal populations. The targets of MC192-Saporin are p75NTR-expressing cells including cholinergic neurons of the basal forebrain, cerebellar Purkinje cells, medial septum, diagonal band of Broca, Nucleus basalis of Meynert and some tumour cells. MC192-saporin has been used in the study of learning and memory and its primary application is in vivo, MC192-saporin is specific for applications in rat. The antibody does not cross-react with human or mouse p75NTR receptors.
Application Details1. To specifically target and eliminate rat cells expressing p75NTR in vivo. MC192-saporin has been used to selectively lesion cholinergic neurons of basal forebrain to create an animal model to study Alzheimer's disease. 2. To be used as a model for gene delivery into neurons.
Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetNGFR/p75 neurotrophin receptor (p75NTR)
SpecificityMC192 antibody is specific only for rat NGFR, no reactivity to human or mouse NGFR has been reported This monoclonal antibody does not cross react with p75NTR-expressing cells in other species than rat.
Target Host SpeciesRat
Species ReactivityRat
Antibody HostMouse
Antibody TypeMonoclonal
Antibody IsotypeIgG1
Clone NameMC192
ConjugateSaporin
Immunogen DescriptionRat NGF receptor (p75NTR)
Biological ActivityActive. Ablates p75-positive cells in rat in vivo. Routinely tested for dose-dependent killing of rat C6 cells in vitro. Note that the primary use of MC192-saporin is for in vivo applications in rat. Effective MC192-saporin concentrations must be determined for every new batch.
Purity DescriptionConjugate was purified by ion-exchange chromatography. Purity > 90% by non-reducing SDS-PAGE
FormatLyophilized from a 1 mg/mL solution containing PBS pH 7.2-7.6 without preservative.
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in sterile-filtered, ultrapure water to achieve a conjugate concentration of 1 mg/mL. Centrifuge to remove any insoluble material. Final buffer contains no preservatives.
Storage InstructionsLyophilized product is shipped at ambient room temperature. Upon receipt, pulse-centrifuge the vial to collect solid that may be entrapped in the lid. After reconstitution, immediately prepare aliquots and keep the undiluted stock at -80°C for long-term storage. Avoid repeated thaw-freezing. For short-term storage, keep at 2-8°C for up to 2 weeks. it is recommended to handle this product under sterile conditions.
Hazardous StatementOnly qualified personnel should handle this product. MC192 antibody does not bind to human p75NTR receptor, however, standard laboratory protective measures should be followed, including wearing lab coat, gloves and safety glasses. Avoid contact with open wounds and practice good laboratory technique to avoid auto-exposure/auto-injection! Avoid contact of conjugate with reducing agents (eg., DTT, beta-mercaptoethanol) as this will destroy the antibody-saporin conjugate.
Immunohistochemical staining of p75NTR in rat forebrain. (A) Brain section from a control rat. (B) Brain section from a rat injected intraventricularly with 4 µg of 192-Saporin. Seven days after the treatment the rat was perfused with 4% paraformaldehyde and free floating section at 40 µm was used for staining.