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Oxidized low-density lipoprotein receptor 1 (Lox-1/OLR1), Human, ELISA assay
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Catalog Number
BEK-2347
- Product Name Oxidized low-density lipoprotein receptor 1 (Lox-1/OLR1), Human, ELISA assay
- Product Description The Human OLR1 ELISA kit is a sandwich ELISA. The capture antibody is a mouse monoclonal OLR1 antibody pre-coated onto the 96-well strip plates provided in the kit. Rat test samples and standards of known OLR1 concentration are added to these wells and allowed to complex with the bound OLR1 antibody. A biotinylated OLR1 goat polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of OLR1F present in the samples. The purpose of this kit is the in-vitro quantitative determination of human OLR1 in samples such as culture supernatant, cell lysates, serum, plasma (Citrate), plasma (EDTA) and plasma (Heparin). This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
- Alternative Names Oxidized low-density lipoprotein receptor 1; Lox-1; C-type lectin domain family 8 member A; Lectin-like oxLDL receptor 1; hLOX-1; Lectin-type oxidized LDL receptor 1; OLR1;
- Application(s) ELISA
- Specificity Human OLR1
- Species Reactivity Human
- Immunogen Description The standard and immunogen for this kit is recombinant human OLR1. The expression system is NSO; Immunogen sequence: S61-Q273
- Range 31.2 pg/mL - 2,000 pg/mL
- Sensitivity < 10 pg/mL
- Regulatory Status For research use only.
Product Info
- Product Description The Human OLR1 ELISA kit is a sandwich ELISA. The capture antibody is a mouse monoclonal OLR1 antibody pre-coated onto the 96-well strip plates provided in the kit. Rat test samples and standards of known OLR1 concentration are added to these wells and allowed to complex with the bound OLR1 antibody. A biotinylated OLR1 goat polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of OLR1F present in the samples. The purpose of this kit is the in-vitro quantitative determination of human OLR1 in samples such as culture supernatant, cell lysates, serum, plasma (Citrate), plasma (EDTA) and plasma (Heparin). This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
- Application(s) ELISA
- Application Details ELISA. For the quantification of Oxidized low-density lipoprotein receptor 1 (Lox-1/OLR1) in Culture Supernatant, Cell Lysates, Serum, Plasma (Heparin), Plasma (EDTA). Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
- Target Oxidized low-density lipoprotein receptor 1 (Lox-1/OLR1)
- Specificity Human OLR1
- Target Cross-Reactivity (ELISA) There is no detectable cross-reactivity with other relevant proteins.
- Target Host Species Human
- Species Reactivity Human
- Immunogen Description The standard and immunogen for this kit is recombinant human OLR1. The expression system is NSO; Immunogen sequence: S61-Q273
- Range 31.2 pg/mL - 2,000 pg/mL
- Sensitivity < 10 pg/mL
- Sample Type Cell Lysates, Culture Supernatant, Plasma (EDTA), Plasma (Heparin), Serum
- Detection Method Colorimetric
- Kit Components The ELISA kit box contains 96-well pre-coated strip plate(s), human OLR1 protein standards, human OLR1 biotinylated antibody, Avidin-Biotin-Peroxidase Complex, sample diluent, antibody and avidin-biotin peroxidase diluents, substrate reagent, stop solution, wash buffer, plate sealers and detailed protocols.
- Storage Instructions Store at 2-8°C
- Storage Temperature 2-8°C
- Batch Number Please see item label.
- Expiration Date 6 months after date of receipt if unopened
- Alternative Names Oxidized low-density lipoprotein receptor 1; Lox-1; C-type lectin domain family 8 member A; Lectin-like oxLDL receptor 1; hLOX-1; Lectin-type oxidized LDL receptor 1; OLR1;
- Uniprot Number P78380
- Uniprot Number/Name P78380 (OLR1_HUMAN)
- Scientific Background LOX-1/OLR1, is a receptor protein that in humans is encoded by the OLR1 gene. LOX-1 mediates the recognition, internalization and degradation of oxidatively modified low density lipoprotein (oxLDL) by vascular endothelial cells. OxLDL is a marker of atherosclerosis that induces vascular endothelial cell activation and dysfunction, resulting in pro-inflammatory responses, pro-oxidative conditions and apoptosis (Uniprot).
- Shipping Statement For order quantities larger than 1 plate, this product is supplied in package units of 2 plates.
- Shipping Temperature 2-8°C (on cold packs)
- UNSPSC CODE 41116158
- Regulatory Status For research use only.
