Product DescriptiongoogleThe mouse Oxidized low-density lipoprotein receptor 1 (Ox-LDL receptor 1) Kit is a sandwich ELISA. The capture antibody is a monoclonal mouse Ox-LDL receptor 1 antibody pre-coated onto the 96-well strip plates provided in the kit. Test samples and standards of known Ox-LDL receptor 1 concentration are added to these wells and allowed to complex with the bound Ox-LDL receptor 1 antibody. A biotinylated anti-Ox-LDL receptor 1 antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of Ox-LDL receptor 1 present in the samples. The purpose of this kit is the in-vitro quantitative determination of mouse Ox-LDL receptor 1 in samples such as culture supernatant, cell lysates, serum, plasma (Heparin) and plasma (EDTA). This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Alternative NamesOx-LDL receptor 1; C-type lectin domain family 8 member A; Lectin-like oxidized LDL receptor 1; LOX-1; Lectin-like oxLDL receptor 1; hLOX-1; Lectin-type oxidized LDL receptor 1; LOX1; OLR1; CLEC8A;LOX-1/OLR1
Immunogen DescriptionThe standard and immunogen used for this LOX-1/OLR1 ELISA is recombinant mouse LOX-1/OLR1 amino acids 60-363 that was produced in and purified from a mammalian cell expression system.
Product DescriptionThe mouse Oxidized low-density lipoprotein receptor 1 (Ox-LDL receptor 1) Kit is a sandwich ELISA. The capture antibody is a monoclonal mouse Ox-LDL receptor 1 antibody pre-coated onto the 96-well strip plates provided in the kit. Test samples and standards of known Ox-LDL receptor 1 concentration are added to these wells and allowed to complex with the bound Ox-LDL receptor 1 antibody. A biotinylated anti-Ox-LDL receptor 1 antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of Ox-LDL receptor 1 present in the samples. The purpose of this kit is the in-vitro quantitative determination of mouse Ox-LDL receptor 1 in samples such as culture supernatant, cell lysates, serum, plasma (Heparin) and plasma (EDTA). This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Application(s)ELISA
Application DetailsELISA. For the quantification of Oxidized low-density lipoprotein receptor 1, soluble (sLOX-1/OLR1) in Culture Supernatant, Cell Lysates, Serum, Plasma (EDTA), Plasma (Heparin). Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
Immunogen DescriptionThe standard and immunogen used for this LOX-1/OLR1 ELISA is recombinant mouse LOX-1/OLR1 amino acids 60-363 that was produced in and purified from a mammalian cell expression system.
Kit ComponentsThe ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, detection reagents, substrate buffer and detailed protocols.
Storage InstructionsStore at 2-8°C
Storage Temperature2-8°C
Batch NumberPlease see item label.
Expiration DateThis kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at –80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
Alternative NamesOx-LDL receptor 1; C-type lectin domain family 8 member A; Lectin-like oxidized LDL receptor 1; LOX-1; Lectin-like oxLDL receptor 1; hLOX-1; Lectin-type oxidized LDL receptor 1; LOX1; OLR1; CLEC8A;LOX-1/OLR1