Product DescriptiongoogleGuinea Pig anti-Oxytoxin Polyclonal Antibody (Unconjugated), suitable for IHC-Frozen.
Alternative NamesOT; OXT;OT-NPI; OT-NPI;
Application(s)IHC-Frozen
Antibody HostGuinea Pig
Antibody TypePolyclonal
SpecificitySpecificity was demonstrated by immunohistochemistry. This antibody is known to react with sheep. Other species have not yet been tested.
Species ReactivityHuman, Sheep
Immunogen DescriptionA synthetic peptide (CYIQNCPLG) corresponding to the amino acids 20-28 from human Oxytocin-neurophysin 1. This peptide was conjugated to carrier protein to enhance the immunological response.
Product DescriptionGuinea Pig anti-Oxytoxin Polyclonal Antibody (Unconjugated), suitable for IHC-Frozen.
Application(s)IHC-Frozen
Application DetailsIHC. A concentration of 1 in 2000 is recommended for IHC. IHC performed in sheep brain (hypothalamus) demonstrates intense staining of cells and terminals. No staining is evident when the primary antibody is pre-absorbed with 0.5 mg/mL of oxytocin. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetOxytoxin
SpecificitySpecificity was demonstrated by immunohistochemistry. This antibody is known to react with sheep. Other species have not yet been tested.
Target Host SpeciesHuman
Species ReactivityHuman, Sheep
Antibody HostGuinea Pig
Antibody TypePolyclonal
Antibody IsotypeMixed
ConjugateUnconjugated
Immunogen DescriptionA synthetic peptide (CYIQNCPLG) corresponding to the amino acids 20-28 from human Oxytocin-neurophysin 1. This peptide was conjugated to carrier protein to enhance the immunological response.
Purity DescriptionNeat serum.
FormatLyophilized
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 50 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material.
Storage InstructionsIt is recommended that a thawed sample is stored at 2-8°C for no longer than 2 weeks. Allocation of appropriate anti-bacterial agent can increase shelf life by several weeks. Diluted serum should be prepared as required. Long term stability requires storage, preferably in small aliquots at -20°C or lower. Glycerol (1:1) can be added to neat serum for additional stability if intended use does not prevent this.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Scientific BackgroundOxytocin causes contraction of the smooth muscle of the uterus and of the mammary gland. It belongs to the vasopressin/oxytocin family. Oxytocin is secreted.
The cryostat section of the sheep hypothalamus was incubated in guinea pig polyclonal antibodies to oxytocin at the dilution of 1:1000 overnight followed by incubation with biotinylated secondary antibodies. Cell bodies and nerve terminals (from cells that produce this hormone) in the sheep brain are intensely stained. This figure shows staining of cells when no pre-absorption is performed.
Immunofluorescent visualization of magnocellular oxytocin neurons in formalin-fixed floated cryostat section from the human paraventricular nucleus. The oxytocin antiserum (gp-037-50; 1:3,000) was reacted with Cy3-conjugated anti-guinea pig antibodies. Photo courtesy of Dr. Erik Hrabovszky, Hungarian Academy of Sciences, Budapest, Hungary.
Immuno-peroxidase detection of oxytocin neurons in the human hypothalamic supraoptic nucleus, using guinea pig polyclonal oxytocin antibodies (gp-037-50; 1:50,000) with the biotinylated secondary antibody-ABC technique and Ni-DAB chromogen. Photo courtesy of Dr. Erik Hrabovszky, Hungarian Academy of Sciences, Budapest, Hungary.
Immuno-peroxidase detection of oxytocin neurons in the hypothalamic paraventricular nucleus of the rat, using guinea pig polyclonal oxytocin antibodies (gp-037-50; 1:50,000) with the biotinylated secondary antibody-ABC technique and Ni-DAB chromogen. Photo courtesy of Dr. Erik Hrabovszky, Hungarian Academy of Sciences, Budapest, Hungary.
General ReferencesThompson MR, Callaghan PD, Hunt GE, Cornish JL, Mcgregor IS. (2007). A role for oxytocin and 5-HT1A receptors in the prosocial effects of 3,4 Methylendioxymethamphetamine (ecstasy). Neuroscience. 146(2) pp. 509-14.
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