Pentraxin-related protein PTX3 (Human PTX3), Human, ELISA assay

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Catalog Number
BEK-2372

    Product Info

  • Product Name Pentraxin-related protein PTX3 (Human PTX3), Human, ELISA assay
  • Product Description google The Human Pentraxin-related protein PTX3 (Human PTX3) Kit is a sandwich ELISA. The capture antibody is a monoclonal antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known Human PTX3 concentration are added to these wells and allowed to complex with the bound Human PTX3 antibody. A biotinylated Human PTX3 polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of Human PTX3 present in the samples. There is no significant cross-reactivity between Human PTX3 and its analogs, however cross-reactivity may exist with untested analogs. The purpose of this kit is the in-vitro quantitative determination of Human IFN-lambda-2 in culture supernatant, serum and plasma (heparin, EDTA) and saliva.This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
  • Alternative Names Human Pentraxin-related protein PTX3, PTX3, Tumor necrosis factor alpha-induced protein 5, TNF alpha-induced protein 5, TSG-14, Tumor necrosis factor-inducible gene 14 protein, TNFAIP5
  • Application(s) ELISA
  • Specificity This Human Pentraxin-related protein PTX3 ELISA assay is reactive to Human PTX3 in human samples. There is no detectable cross-reactivity with other relevant proteins.
  • Species Reactivity Human
  • Immunogen Description Expression system for standard: NS0; Immunogen sequence: E18-S381
  • Range 312 pg/mL – 20,000 pg/mL
  • Sensitivity < 10 pg/mL
  • Regulatory Status For research use only.

    Specifications

  • Product Description The Human Pentraxin-related protein PTX3 (Human PTX3) Kit is a sandwich ELISA. The capture antibody is a monoclonal antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known Human PTX3 concentration are added to these wells and allowed to complex with the bound Human PTX3 antibody. A biotinylated Human PTX3 polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of Human PTX3 present in the samples. There is no significant cross-reactivity between Human PTX3 and its analogs, however cross-reactivity may exist with untested analogs. The purpose of this kit is the in-vitro quantitative determination of Human IFN-lambda-2 in culture supernatant, serum and plasma (heparin, EDTA) and saliva.This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
  • Application(s) ELISA
  • Application Details ELISA. For the quantification of Human Pentraxin-related protein PTX3 in Culture Supernatant, Serum, Plasma (EDTA), Plasma (Heparin) and Saliva. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
  • Target Pentraxin-related protein PTX3 (Human PTX3)
  • Specificity This Human Pentraxin-related protein PTX3 ELISA assay is reactive to Human PTX3 in human samples. There is no detectable cross-reactivity with other relevant proteins.
  • Target Cross-Reactivity (ELISA) There is no detectable cross-reactivity with other relevant proteins.
  • Target Host Species Human
  • Species Reactivity Human
  • Immunogen Description Expression system for standard: NS0; Immunogen sequence: E18-S381
  • Range 312 pg/mL – 20,000 pg/mL
  • Sensitivity < 10 pg/mL
  • Sample Type Culture Supernatant, Plasma (EDTA), Plasma (Heparin), Saliva, Serum
  • Detection Method Colorimetric
  • Kit Components The ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, detection reagents, substrate buffer and detailed protocols.
  • Storage Instructions Store at 2-8°C
  • Storage Temperature 2-8°C
  • Batch Number Please see item label.
  • Expiration Date This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at –80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
  • Alternative Names Human Pentraxin-related protein PTX3, PTX3, Tumor necrosis factor alpha-induced protein 5, TNF alpha-induced protein 5, TSG-14, Tumor necrosis factor-inducible gene 14 protein, TNFAIP5
  • Uniprot Number P26022
  • Uniprot Number/Name P26022 PTX3_HUMAN
  • Scientific Background Plays a role in the regulation of innate resistance to pathogens, inflammatory reactions, possibly clearance of self-components and female fertility. Reference: uniprot.org
  • Shipping Statement For order quantities larger than 1 plate, this product is supplied in package units of 2 plates.
  • Shipping Temperature 2-8°C (on cold packs)
  • UNSPSC CODE 41116158
  • Regulatory Status For research use only.

    Citations & References