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Bovine A1 Beta Casein ELISA Kit (1 plate)

$397.00USD


Catalogue No. BEK-2243-1P
Description
β-Casein is expressed as 13 genetic variants resulting from single nucleotide polymorphisms (SNP) in the CSN2 gene. The most frequent genetic variants in western dairy breeds are β-Casein A1 and β-Casein A2. The two types of β-Casein protein, A1 and A2, differ by a single-point mutation at amino acid position 82 (P82/H82).

The Biosensis Bovine A1 β-Casein enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the quantification of the bovine A1 β-Casein isoform in 5 hours. This kit consists of a pre-coated rabbit anti-Bovine β-casein polyclonal capture antibody, a chicken anti-bovine A1 β-casein detection antibody and a horseradish peroxidase (HRP)-conjugated donkey anti-chicken IgY antibody. The addition of a substrate (3,3,5,5-tetramethylbenzidine, TMB) yields a coloured reaction product which is directly proportional to the concentration of Bovine A1 β-Casein present in samples and protein standards.

Extensive validation has shown accurate quantification of A1 β-casein in UHT (Ultra High Temperature) treated milk or long life milk, organic pasteurized and homogenized full cream milk, fresh pasteurized and homogenized milk, cold-pressed raw milk (non-pasteurized, non-homogenized), and biodynamic full-cream whole milk. Please refer to the kit protocol for specific use instructions.

The A2 isoform is not detected in this ELISA assay.
Related products BEK-2364-SET5, Bovine A1 Beta Casein ELISA Kit Bulk Pack (5 plates)
BEK-2364-SET10, Bovine A1 Beta Casein ELISA Kit Bulk Pack (10 plates)
BEK-2244-1P, Bovine A2 Beta Casein ELISA Kit (1 Plate)
BEK-2365-SET5, Bovine A2 Beta Casein ELISA Kit Bulk Pack (5 plates)
BEK-2365-SET10, Bovine A2 Beta Casein ELISA Kit Bulk Pack (10 plates)
Batch No. Refer to the product label.
Antigen Bovine A1 beta casein (H82).
Accession P02666 (CASB_BOVIN)
Specificity Bovine A1 beta-casein.
Cross-reactivity No cross-reactivity is observed with bovine A2 beta-Casein
Storage Store at 2-8C.
Expiry Date 12 months from purchase.
Kit components The ELISA kit box contains 1 x 96-well pre-coated strip plate, protein standards, detection reagents, wash and sample buffers, substrate buffer, stop solution and detailed protocols.
Range 3.1 ng/mL - 200 ng/mL
Sensitivity This bovine A1 beta-casein ELISA kit detects a minimum of 3 ng/mL A1 beta-casein in assay buffer (defined as A1 concentration at blank OD plus 3x standard deviations of the blank OD (n=10))
Kit protocol Please contact us at biospeak@biosensis.com for a detailed protocol for this ELISA kit.
Images (click to zoom)
Bovine A1 Beta Casein ELISA Kit (1 plate) Parallelism in bovine milk samples demonstrates accurate quantification of A1 beta-casein.
Bovine A1 Beta Casein ELISA Kit (1 plate) This standard curve generated in our laboratories is for demonstration purposes only, but can be used as a guide to expected performance. A standard curve should be generated for each assay.
Bovine A1 Beta Casein ELISA Kit (1 plate) Bovine A2 beta-casein does not cross-react in this ELISA assay.
PDF Data Sheet

Click to download the data sheet - Click to download the Data Sheet

MSDS

Click to download the MSDS - Click to download the MSDS

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