| Catalogue No. |
PE-1832-20 |
| Description |
Bovine milk may contain two types of beta-casein protein, A2 or A1, which differ by a single-point mutation at amino acid position 82 (P82/H82). Recent studies have shown that milk containing the A1 beta casein protein can contribute to issues including gastrointestinal discomfort after ingestion. There is some evidence of a link between ingestion of A1 beta casein protein and the development of Type 1 diabetes.
PE-1832-20 is A2 beta casein protein that is purified in its native form from bovine A2/A2 milk to >95% purity. The milk is collected from commercial cows certified free of disease and BSE by the manufacturer of the milk.
IMPORTANT: This product may give different OD readings as compared to the protein calibrator contained in our A2 Casein ELISA Kit due to its formulation. Thus this product is not recommended as a standard or QC marker for the A2 ELISA assay. |
| Related products |
BEK-2365-1P, Bovine A2 Beta Casein ELISA Kit (1 plate)
C-1780-100, Chicken antibody to bovine A2 Beta Casein Protein: Affinity purified |
| Batch No. |
Refer to the product label. |
| Unit size |
20 ug |
| Sequence |
Bovine A2 beta casein differs from A1 beta casein at amino acid position 82 (P82) |
| Other Names |
Beta Casein |
| Accession |
P02666 (CASB_BOVIN) |
| Produced in |
Affinity-purified from organic, natural bovine A2/A2 milk. |
| Molecular Weight |
~33 kDa (SDS-PAGE) |
| Purity |
>95% (SDS-PAGE) |
| Biol. activity |
Not tested |
| Applications |
Western blotting (100 ng or less) as a standard, not recommended for ELISA. Other applications not yet tested. |
| Specificity |
Bovine A2 beta casein protein only binds A2-specific antibody as determined by Western Blotting. Protein runs as a single band (> 95%) with very few minor bands (< 5%) on SDS-PAGE gels. |
| Form |
Affinity-purified, native protein. |
| Appearance |
Liquid. Protein is provided as 50 uL aliquot at 0.4 mg/mL in 10 mM borate buffer, pH 8.5, containing 20% glycerol. Does not contain preservatives. Use sterile methods. |
| Storage |
Protein is shipped on ice packs. Upon receipt, aliquot and store product long-term at -20C. Store working aliquots short-term at 2-8C as undiluted liquid for one week. AVOID MULTIPLE FREEZE THAWS. |
| Expiry Date |
12 months from purchase if stored at -20C. |
