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Chicken antibody to GFR alpha-3: affinity purified


Catalogue No. C-1661-100
Description The GFRa-3 is a glycosylphosphatidylinositol(GPI)-linked cell surface receptor and a member of the GDNF receptor family. It forms a signaling receptor complex with RET tyrosine kinase receptor and binds the ligand, artemin.
Related products R-068-100 Rabbit antibody to GFR alpha-3 (1-11): whole serum;C-1659-100 Chicken antibody to GFR alpha-1: affinity purified;C-1660-100 Chicken antibody to GFR alpha-2: affinity purified;C-1662-100 chicken antibody to GFR alpha-4: affinity purified;
Batch No. See product label
Unit size 100 ug
Antigen Recombinant His-tagged human GFRa-3 protein produced using CHO-based cell line. For production of hGFRa-3, glycosylphosphatidyl-inositol GPI-anchor was removed and protein was secreted to the cell culture supernatant. Protein was purified by Ni-affinity chromatography following gel-filtration from cell culture supernatant.
Sequence Uniprot O60609
Antibody Type Polyclonal
Isotype IgY
Other Names GDNF receptor alpha-3
Accession O60609
Produced in Chicken
Purity Affinity purified
Applications WB, ICC. A dilution of 1:5 000 to 1:10 000 is recommended for Western blot and 1:1500 to1:3000 for immunocytochemistry. Biosensis recommends that optimal dilutions/concentrations should be determined by the end user.
Specificity Human GFRa-3. No cross-reactivity with hGFRa-1, hGFRa-2, hGFRa-4.
Species Against Human
Blast it If you would like to use this product in another species other than those specified here, or to see the shared ID between the immunogen used here in different speices and/or other molecules, simply copy the immunogen (from the Immunogen field) and paste it HERE and blast/format it. Note that, antisera raised against synthetic peptides are quite often very specific for that peptide ie, only one single amino acid difference may be enough to restrict the specificity to a particular molecule. Regardless, you can always contact us if you need assistance with this.
Form Concentrated ammonium sulphate in PBS pH 7.4 contain no preservatives
Reconstitution Store antibody stock solution at 2-8C upon receipt. DO NOT FREEZE. This product is an (NH4)2SO4 precipitate. To use, mix well by pipetting or vortexing gently prior use, then withdraw the desired amount of antibody stock solution and dilute into your antibody dilution buffer and use normally.
Alternatively, material can be centrifuged at 10K x g for 5 minutes and the liquid sulfate solution carefully removed. Then precipitated antibody can be resuspended in 0.1M P, 250mM NaCL, pH 7.4 solution. The solution should then be divided into working aliquots and stored at -80C until used. Prevent multiple freeze thaw cycles of aliquots for best product life.
Storage Store at 2-8C upon receipt; DO NOT FREEZE. As product is (NH4)2SO4 (ammonium sulfate) precipitate, mix well by pipetting or vortexing prior to use. See reconstitution instructions for more information.
Expiry Date 12 months after purchase.
Images (click to zoom)
Chicken antibody to GFR alpha-3: affinity purified

Western-Blot detection of human GFRα-3, using Chicken polyclonal antibody to human GFRα-3. GFRα-3 was C-terminally His tagged. Protein was expressed by CHO cell culture. 100 ng of purified protein was loaded per lane. Line 1. Prestained protein ladder (Naxo 8003). Line 2. hGFRα-1. Primary antibody dilution 1:5000 was used.

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