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Chicken antibody to GFR alpha-4: affinity purified


Catalogue No. C-1662-100
Description GFRa-4 belongs to the GDNF receptor family. It is a glycosyl-phosphatidylinositol (GPI)-linked cell surface receptor for persephin, and mediates activation of the RET tyrosine kinase receptor.
Related products C-1659-100 Chicken antibody to GFR alpha-1: affinity purified;C-1660-100 Chicken antibody to GFR alpha-2: affinity purified;C-1661-100 Chicken antibody to GFR alpha-3: affinity purified;
Batch No. See product label
Unit size 100 ug
Antigen Recombinant His-tagged human GFRa-4 protein produced using CHO-based cell line. For production of hGFRa-4, glycosylphosphatidyl-inositol GPI-anchor was removed and protein was secreted to the cell culture supernatant. Protein was purified by Ni-affinity chromatography following gel-filtration from cell culture supernatant.
Sequence Uniprot Q9GZZ7
Antibody Type Polyclonal
Isotype IgY
Other Names GDNF receptor alpha-4, Persephin receptor
Accession Q9GZZ7
Produced in Chicken
Purity Affinity purified
Applications WB, IHC. A dilution of 1:5 000-1:10 000 is recommended for Western blots and 1:1 000 to 1:3 000 for immunocytochemistry. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Specificity Human GFRa-4. This antibody does not cross-react with hGFRa-1, hGFRa-2, hGFRa-3
Species Against Human
Antibody Against GFR alpha-4
Blast it If you would like to use this product in another species other than those specified here, or to see the shared ID between the immunogen used here in different speices and/or other molecules, simply copy the immunogen (from the Immunogen field) and paste it HERE and blast/format it. Note that, antisera raised against synthetic peptides are quite often very specific for that peptide ie, only one single amino acid difference may be enough to restrict the specificity to a particular molecule. Regardless, you can always contact us if you need assistance with this.
Form Liquid in PBS pH 7.4 containing no preservatives
Reconstitution Ready to dilute once thawed
Storage Store at -80C upon receipt. Divide antibody into aliquots upon receipt. Avoid multiple freeze-thaw cycles as product degradation may result.
Expiry Date 12 months after purchase.
Images (click to zoom)
Chicken antibody to GFR alpha-4: affinity purified

Western-Blot detection of human GFRα-4, using Chicken polyclonal antibody to human GFRα-4. GFRα-4 was expressed by CHO cell culture. 100 ng of purified protein was loaded per lane. Line 1. hGFRα-4. Primary antibody dilution 1:5000 was used. Line 2.  Prestained protein ladder (Naxo 8003).

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