||Bovine milk contains two types of beta-casein protein, A2 or A1. Recent studies have shown that milk containing the A1 beta casein protein can contribute to issues including gastrointestinal discomfort after ingestion. There is some evidence of a link between ingestion of A1 beta casein protein and the development of Type 1 diabetes.
|| C-1781-100: Chicken antibody to bovine A1 beta casein: Affinity purified.
R-1794-100: Rabbit antibody to bovine beta casein: Affinity purified.
||Please refer to the product label.
||100 uL (Refer to label for antibody concentration)
||A synthetic peptide (PGPIPNSLP, aa: 78-86) conjugated to KLH has been used as immunogen. Bovine A1 beta casein differs from bovine A2 beta casein by one amino acid (H82 -> P82)
||Affinity purified chicken IgY.
||Western blot and ELISA. Suggested working dilution for western blot is 1:200-1:1,000. The amount of milk per lane can be 0.05ul-0.1ul for Western blot. Sample Preparation: Milk should be diluted 1:10 in 0.1M NaOH. The reason for diluting (1:10) in 0.1M NaOH is because the milk protein is easier to dissolve in NaOH. The sample is then further diluted with PBS or other buffer and mixed at 1:1 ratio, to prepare for loading. For example, one can take the 1:10 dilution milk (0.5ul-1ul) and add into 9.5ul or 9ul PBS and mix with 10ul SDS-PAGE Sample buffer, boil for 5 minutes, quick spin, and load on the gel. Recommended blocking buffer: TBS with 5% BSA. Recommended antibody dilution buffer: TBST containing 3% BSA. Biosensis recommends that optimal working dilutions should be determined by the end user.
||The antibody is specific to A2 beta casein by western blot and ELISA. No cross-reactivity with A1 beta casein is seen.
||Species cross-reactivity not tested.
||Liquid in PBS, pH 7.4, containing 0.02% sodium azide as preservative. Refer to the product label for antibody concentration.
||Maintain unopened vial at -20C for up to 12 months after date of receipt. Prior to opening it is recommended that the tube is microfuged for a few seconds to insure that all liquid is in the bottom of the vial. After opening maintain at -20C in undiluted aliquots for up to 6 months. For short-term storage, keep aliquot at 2-8C for up to one week. Avoid repeated freeze-thaw cycles.
||12 months after date of receipt unopened and stored at -20C.
||Nutr Diabetes. 2017 May 15;7(5):e274
Nutr J. 2016 Apr 2;15:35