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Chicken antibody to human nestin: IgY


Catalogue No. C-1812-50
Description Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells (By similarity). Ref:
Batch No. See product label.
Unit size 50 uL
Antigen Part of recombinant human protein (amino acids 315-630).
Accession P48681 (NEST_HUMAN)
Produced in Chicken
Purity IgY fraction
Applications Western blotting (1:1,000-1:5,000) and Immunocytochemistry (1:2,000-1:5,000). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Specificity Reacts with human and rat. Other species not tested.
Form Lyophilised from a solution containing PBS buffer pH 7.2-7.6, without preservatives.
Reconstitution Spin vial briefly before opening. Reconstitute in 50 uL sterile water. Centrifuge to remove any insoluble material. Final buffer contains no preservatives.
Storage Store lyophilised antibody at 2-8C. After reconstitution divide into aliquots and store at -20C for long-term storage. Store at 2-8C short-term (up to 4 weeks) with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles.
Expiry Date 12 months after purchase if unopened.
Images (click to zoom)
Chicken antibody to human nestin: IgY Left: SH-SY5Y neuroblastoma cells stained with chicken antibody to Nestin (green, 1:2,000) and rabbit antibody to Vimentin (R-1699-100, red, 1:2,000) by Immunocytochemistry. Vimentin is main partner of nestin forming heterodimers, further polymerizing to form intermediate filaments. Co-localization of vimentin and nestin in cells is shown in yellow. Blue: DAPI staining of nuclear DNA. Right: Western blot analysis of nestin expression in rat C6 (Lane 1) and human SH-SY5Y (Lane 2) cell lysates. The antibody (1:1,000) detects one specific band running at ~260 kDa corresponding to full-length nestin protein.
Chicken antibody to human nestin: IgY Nestin staining (red) in human mesenchymal stem cells by Immunocytochemistry. Primary antibody dilution: 1:3,000. Image courtesy of QBM Cell Science.
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