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Chicken antibody to human Ki-67: IgY


Catalogue No. C-1807-50
Description Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly (PubMed:27362226). Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface (PubMed:27362226). Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility (PubMed:27362226). Binds DNA, with a preference for supercoiled DNA and AT-rich DNA (PubMed:10878551). Does not contribute to the internal structure of mitotic chromosomes (By similarity). May play a role in chromatin organization (PubMed:24867636). It is however unclear whether it plays a direct role in chromatin organization or whether it is an indirect consequence of its function in maintaining mitotic chromosomes dispersed (Probable). Ref:
Batch No. See product label.
Unit size 50 uL
Antigen Recombinant human Ki-67 protein (mixture of amino acids 1-300 and 1,111-1,490) expressed in and purified from E. coli.
Other Names Proliferation marker protein Ki-67; Antigen identified by monoclonal antibody Ki-67; Antigen KI-67; Antigen Ki67
Accession P46013 (KI67_HUMAN)
Produced in Chicken
Purity IgY fraction
Applications Western blotting (1:2,000-1:5,000) and Immunocytochemistry (1:1,000-1:5,000). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Specificity Reacts with human only. Does not react with mouse or rat.
Form Lyophilised from a solution containing PBS buffer pH 7.2-7.6, without preservatives.
Reconstitution Spin vial briefly before opening. Reconstitute in 50 uL sterile water. Centrifuge to remove any insoluble material. Final buffer contains no preservatives.
Storage Store lyophilised antibody at 2-8C. After reconstitution divide into aliquots and store at -20C for long-term storage. Store at 2-8C short-term (up to 4 weeks) with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles.
Expiry Date 12 months after purchase if unopened.
Images (click to zoom)
Chicken antibody to human Ki-67: IgY Left: Immunofluorescence analysis of HeLa cells costained with chicken antibody to Ki-67 (1:2,000, red) and a mouse antibody to beta-tubulin. Ki-67 is predominantly localized in nucleoli of cells in interphase, while in mitotic cells Ki-67 is associated with condensed chromosomes. Ki-67 is not expressed in cells in quiescent G0 state. Blue: DAPI staining of nuclear DNA. Right: Western blot analysis of equal amounts of HeLa (Lane 2) and HEK293 (Lane 3) cell lysates using chicken antibody to Ki-67 (1:5,000). Double bands above 250 kDa correspond to two major Ki-67 isoforms of molecular weight ~345 and 395 kDa. Smaller fragments of these isoforms are also detected on the blot.

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