| Catalogue No. |
C-1373-50 |
| Description |
Glial fibrillary acidic protein (GFAP) is approx. 50 kDa intra-cytoplasmic filamentous protein of the cytoskeleton in astrocytes. During the development of the central nervous system, it is a cell-specific marker that distinguishes astrocytes from other glial cells. GFAP immunoreactivity has been shown in immature oligodendrocytes, epiglottic cartilage, pituicytes, papillary meningiomas, myoepithelial cells of the breast and in non-CNS: Schwann cells, salivary gland neoplasms, enteric glia cells, and metastasizing renal carcinomas. |
| Batch No. |
See product label |
| Unit size |
50 uL |
| Antigen |
Recombinant GFAP (expressed in E.coli) and native bovine GFAP |
| Isotype |
IgY |
| Other Names |
Astrocyte; Glial fibrillary acidic protein; GFAP |
| Accession |
P14136 GFAP_HUMAN; Q28115 GFAP_BOVIN; |
| Produced in |
Chicken |
| Molecular Weight |
On western blots of brain or spinal cord homogenate expect to see a band at 55 kDa and another at about 48 kDa, apparently a breakdown product of the primary band. |
| Applications |
Western Blotting (WB), Immunocytochemistry (ICC) and Immunohistochemistry (IHC). A dilution of 1:5,000 is recommended for WB. Human GFAP has a predicted length of 432 residues and a MW of 50 kDa. A dilution of 1:1,000-1:5,000 using fluorescent secondary antibodies or peroxidase or other enzyme-linked methods is recommended for ICC/IHC. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
ICC: 1:1,000-1:5,000, 4% PFA fixed cells in culture, 3hr-o/n incubations, optimization is a must in most cases because of the antibody high reactivity to GFAP
IHC: 4% PFA frozen tissues, permeabilized; IH(P): capable, HEIR treatment typically necessary; Chicken IgY can bind non-specifically to tissues, special chicken block solutions available elsewhere are recommended if used on tissues. Optimization on time, method required for best results. |
| Specificity |
The specificity of this antibody has been confirmed by WB. |
| Antibody Against |
Glial Fibrillary Acidic Protein |
| Cross-reactivity |
Human, Rat, Mouse, Feline. Predicted to react with other mammals. |
| Blast URL |
Click here |
| Form |
Lyophilised with 5% trehalose. The IgY preparation is at a concentration of ~25 mg/mL total protein. |
| Appearance |
White powder |
| Reconstitution |
Reconstitute in sterile distilled water. Centrifuge to remove any insoluble material. |
| Storage |
After reconstitution of lyophilised antibody, aliquot and store at -20C for a higher stability. Avoid freeze-thaw cycles. |
| Expiry Date |
12 months after purchase |
| General References |
1. Brenner M. et al (2001) Mutations in GFAP, encoding glial fibrillary acidic protein, are associated with Alexander disease. Nat Genet. 2001 Jan;27(1):117-20.
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Images (click to zoom)
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Mixed cultures of neurons and glia stained with Chicken polyclonal antibody to Glial Fibrillary Acidic Protein C-1373-50 (red) and DNA (blue). Astrocytes stain strongly and specifically in a clearly filamentous fashion with this antibody. |
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Left: Analysis of GFAP expression (green) in mouse hippocampus section by Immunohistochemistry. Section was stained with chicken anti-GFAP antibody (1:5,000) and co-stained with rabbit antibody to FOX3/NeuN (R-3770-100, 1:5,000, red). Blue: DAPI nuclear stain. IHC Method: Following transcardial perfusion with 4% PFA, mouse brain was post fixed for 24 hours, cut into 45 uM sections, and free-floating sections were stained. The GFAP antibody stains a network of astroglial cells, while the Fox3/NeuN antibody stains the nuclei and proximal perikarya of neurons. Right: Western blot analysis of GFAP expression in whole rat (2) and mouse (3) brain lysates using chicken anti-GFAP antibody (1:5,000). The strong band at about 50 kDa corresponds to GFAP protein. Smaller proteolytic fragments and alternate transcripts of GFAP may also be detected on such blots. |
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