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Chicken polyclonal antibody to Internexin alpha


Catalogue No. C-1377-50
Description Neurofilaments can be defined as the intermediate or 10nm diameter filaments found in neuronal cells. They are composed a mixture of subunits which often includes the neurofilament triplet proteins, NF-L, NF-M and NF-H. Neurofilaments may also include peripherin, alpha-internexin, nestin and in some cases vimentin. Alpha-internexin is a ~66 kDa Class IV intermediate filament subunit expressed in large amounts early in neuronal development, but is downregulated in many neurons as development procedes. Many classes of mature neurons contain alpha-internexin in addition to NF-L, NF-M and NF-H. In some mature neurons alpha-internexin is the only neurofilament subunit expressed. Antibodies to alpha-internexin are therefore unique probes to study and classify neuronal types and follow their processes in sections and in tissue culture. In addition the very early developmental expression of alpha-internexin means its presence is an early and convenient diagnostic feature of neuronal progenitors cells and other cell committed to the neuronal lineage.
Batch No. See product label
Unit size 50 uL
Antigen Recombinant rat alpha-internexin expressed and purified from E. coli
Isotype IgY
Other Names 66 kDa neurofilament protein; NF-66; Neurofilament-66; Alpha-internexin; Alpha-Inx; Neurofilament 5; INA; NEF5;
Accession Q16352 AINX_HUMAN; P23565 AINX_RAT;
Produced in Chicken
Applications Western Blotting (WB), Immunocytochemistry (ICC) and Immunohistochemistry (IHC). A dilution of 1:5,000 - 10,000 is recommended for WB. A dilution of 1:500-1,000 is recommended for ICC and IHC. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Specificity The specificity of this antibody has been confirmed by WB. This antibody is specific for the 64-66 kDa alpha-internexin protein. Molecular weight will depend on species.
Antibody Against Internexin alpha
Cross-reactivity Human, Rat, Mouse, Feline. It is predicted to react with other mammals.
Blast URL Click here
Form Lyophilised with 5% trehalose
Appearance White powder
Reconstitution Reconstitute in sterile distilled water. Centrifuge to remove any insoluble material.
Storage After reconstitution of lyophilised antibody, aliquot and store at -20C for a higher stability. Avoid freeze-thaw cycles.
Expiry Date 12 months after purchase
Images (click to zoom)
Chicken polyclonal antibody to Internexin alpha Mixed neuron glia cultures stain with Chicken polyclonal antibody to Internexin alpha C-1377-50 (red) and counterstained with Rabbit polyclonal antibody to Coronin 1a: Whole serum, R-1335-50 (green) which is an excellent marker of microglia and lymphocytes. This internexin antibody is an excellent marker of neuronal processes in these cultures.
Chicken polyclonal antibody to Internexin alpha Left: Fluorescent staining of rat cerebellum section by Immunohistochemistry with chicken antibody to alpha-internexin (1:5,000, red), and co-stained with mouse antibody to MBP (M-1384-50, 1:5,000, green). IHC Method: Following transcardial perfusion with 4% PFA, brain was post fixed for 24 hours, cut to 45 uM, and free-floating sections were stained. The alpha-internexin antibody selectively stains axons and dendrites of neuronal cells, in particular Purkinje cells, parallel fibers and the axons of granule cells, while the MBP antibody stains myelin sheathes around axons. Right: Western blot analysis of alpha-internexin expression in tissue lysates. [1] protein standard, [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, [6] cow spinal cord, [7] pig spinal cord. The antibody reveals the alpha-internexin protein with apparent molecular weight of 64 to 66 kDa, with some variability among different species. Antibody dilution: 1:10,000.
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