biosensis fine bioscience tools - antibodies, elisa kits, proteins, toxins and peptides An Australia-US partnershipPhone: 1800 605 5127 in the US, or +61 8 8352 7711 for international enquiries to Australia.
HomeAbout BiosensisBiosensis distributorsResourcesHelp deskordering from BiosensisContact usFlyers
 


Technical Notes
 
View our tech notes
 
 
Follow us...
 
Google+ Follow us on Twitter

Find us on Facebook

Sign up for Biosensis newsletters
Visit our newsletter archive
 
 

Fluoro-Jade C (FJC) Powder for identifying Degenerating Neurons

$477.00USD


Catalogue No. TR-160-FJC
Description The causes and effects of neuronal degeneration are of major interest to a wide variety of neuroscientists. Paralleling this growing interest is an increasing number of methods applicable to the detection of neuronal degeneration. Fluoro-Jade C stains all degenerating neurons regardless of specific insult or mechanism of cell death. Fluoro-Jade C exhibits the greatest signal to background ratio, as well as the highest resolution. This translates to a stain of maximal contrast and affinity for degenerating neurons. This makes it ideal for localising not only degenerating nerve cell bodies but also distal dendrites, axons and terminals. The dye is highly resistant to fading and is compatible with virtually all histological processing and staining protocols.

Note: This product is equivalent to discontinued product AG325 from Merck-Millipore.
Related products Fluoro-Jade C (FJC) Ready-to-Dilute Staining Kit for identifying Degenerating Neurons

Fluoro-Jade C (FJC) Ready-to-Dilute Staining Kit for identifying Degenerating Neurons (Trial size)

Fluoro-Jade B (FJB) Powder for identifying Degenerating Neurons
Unit size 30 mg
Applications Following our detailed protocol, Fluoro-Jade C labels degenerating neurons which are visualised with blue light excitation, while DAPI (not included) counter stains cell nuclei, visualised with ultra-violet illumination. The Fluoro-Jade C dye can be used on all kinds of preserved tissues, including fresh-frozen, paraformaldehyde or formalin fixed, and formalin fixed, paraffin-embedded tissues.
Comments MATERIALS PROVIDED

30 mg Fluoro-Jade C, dry powder
Detailed protocol


EQUIPMENT AND REAGENTS NEEDED

Distilled water
ACS grade Ethanol (200 proof) for slide & solution preparation
1% sodium hydroxide in 80% ethanol (basic alcohol solution)
0.1% Acetic Acid solution (in water)
70% ethanol in distilled water
0.06% (KMnO4) potassium permanganate solution
DAPI powder or 100X solution (working range is 0.5-5 µg/mL)
Xylene liquid
Staining dishes/Coplin jars
Cover slips
DPX mounting media or another permanent mounting medium. Non-polar media are preferred over aqueous mounting media such as glycerin/water to obtain high- contrast images (refer to Appendix B in the protocol for a comparative analysis). Traditional fluorescent mounting mediums are not recommended because of their high pH.
Slide warmer
Convection oven
Specificity Degenerating neurons, and neuronal degeneration. There is no specific staining in normal healthy brain.
Cross-reactivity Some researchers under some conditions report blood vessel staining with Fluoro Jade. This may be because Fluoro Jade is an analogue of eosin (which stains blood cells). In general, good perfusion and preparation of the tissue should help prevent blood vessel staining but it may not be possible to eliminate it entirely. In our experience it is generally possible to distinguish neuronal from blood vessels staining by eye.
Form Dry powder.
Appearance FJC visualization is accomplished using blue light or a 488 nm Laser.
Excitation Peak: 495 nm
Emission Peak: 521 nm
Filter system for visualizing: Fluorescein/FITC
Reconstitution Dissolve Fluoro-Jade® C powder in distilled water (10 mg powder per in 100 mL water) to prepare a 0.01% stock solution, filter through a 0.45 µm membrane and store at 4C in the dark for up to 3 months. Discard if cloudy or precipitated. We recommend using aseptic techniques when handling the reagent to avoid bacterial growth and contamination.
Storage The powdered dye can be stored desiccated at room temperature in the dark. Storage in a desiccator is recommended as FJC is hydroscopic. The 0.01% stock solution will remain stable for 3 months when stored in a refrigerator, in the dark. The 0.0001-0.0004% working solution in 0.1% acetic acid should be used within 4 hours of preparation. Diluted FJC dye solutions are not stable and should not be stored. The other diluted solutions can be reused and stored for up to 48 hours if refrigerated and protected from light. Best results require freshly diluted solutions.

The TR-160-FJC material is shipped ambient and stable at room temperature during transport.
Expiry Date The dry powder is stable for 12 months at room temperature if stored as recommended. The 0.1% stock solution can be stored at 2-8C for up to 3 months if handled aseptically. The 0.0001-0.0004% working solution should be used within 4 hours of preparation.
Reagent Kit protocol Click to download the protocolTR-160-FJC_as_at_July2020.pdf
Images (click to zoom)
Fluoro-Jade C (FJC) Powder for identifying Degenerating Neurons Double exposure using combined blue and ultraviolet epi-fluorescent illumination of the superficial layers of the cingulated rat cortex exposed to kainic acid. Layer I contains conspicuous Fluoro-Jade C positive degenerating axon terminals. Layer II contains densely packed DAPI-positive viable granule cells. Layer III contains a mixture of Fluoro-Jade C positive denegerating pyramidal cells and DAPI-positive viable pyramidal cells. Photo is courtesy of Dr. Larry Schmued.
Fluoro-Jade C (FJC) Powder for identifying Degenerating Neurons Triple exposure combining ultraviolet, blue and green light epi-fluorescent illumination (10X) of rat hippocampus exposed to kainic acid. The section was triple labeled with Fluoro-Jade C and DAPI staining combined with GFAP immunohistochemistry. The section reveals extensive green Fluoro-Jade C positive neuronal degeneration throughout the entire CA-1 region of the hippocampus. The underlying blue viable positive granule cells of the dentate gyrus are only DAPI positive. Both regions exhibit red GFAP positive hypertrophied astrocytes. Photo is courtesy of Dr. Larry Schmued.
PDF Data Sheet

Click to download the data sheet - Click to download the Data Sheet

MSDS

Click to download the MSDS - Click to download the MSDS

Offices in the USA and Australia
My Ice Bucket  
 
0 items
 
 
Currencies
 
  
 
 
Email This Page
 
 
Tell someone you know about this product.
 
 
We Accept PayPal
 
You can use secured services of paypal to send us money via our email account MODULE_PAYMENT_PAYPAL_IPN_IDYou can use secured services of paypal to send us money via our email account MODULE_PAYMENT_PAYPAL_IPN_IDYou can use secured services of paypal to send us money via our email account MODULE_PAYMENT_PAYPAL_IPN_ID
Click to make a payment


Official PayPal Seal