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Goat antibody to Cadherin-5 (CDH5, 537-549): Affinity purified

$347.00USD


Catalogue No. G-1928-100
Description Cadherins are calcium-dependent cell adhesion proteins (By similarity). They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types (PubMed:21269602). This cadherin may play a important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions (By similarity). It associates with alpha-catenin forming a link to the cytoskeleton (PubMed:10861224).
Batch No. See product label.
Unit size 100 ug
Antigen A synthetic peptide with sequence C-TVKYGQFDREHTK, from the internal region of the protein sequence according to P33151
Sequence TVKYGQFDREHTK
Antigen Location Internal region
Antibody Type Polyclonal
Other Names CDH5; cadherin 5, type 2 (vascular endothelium); 7B4; CD144; 7B4 antigen; VE-cadherin; cadherin 5, type 2, VE-cadherin (vascular epithelium); cadherin-5; cd144 antigen; endothelial-specific cadherin; vascular endothelial cadherin
Accession P33151 (CADH5_HUMAN)
Produced in Goat
Purity Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Applications Western Blot (1-2 ug/mL), Flow Cytometry (10 ug/mL). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Specificity Preliminary experiments gave an Approx. 100 kDa band in lysates of cell line HeLa and Approx. 110 kDa in A54. Please note that currently we cannot find an explanation in the literature for the band we observed, given the calculated size of 87.5 kDa according to NP_001786.2.
Species Against Human
Cross-reactivity Human
Form Liquid antibody. Supplied at 0.5 mg/mL in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin.
Storage Upon receipt, aliquot and store at -20C long-term. Store at 2-8C short-term (up to 2 weeks). Minimize freezing and thawing.
Expiry Date 12 months after purchase if unopened.
Images (click to zoom)
Goat antibody to Cadherin-5 (CDH5, 537-549): Affinity purified Flow cytometry analysis: of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

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MSDS

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