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Goat antibody to Cadherin 5, type 2 preprotein variant (649-660): Affinity purified

$347.00USD


Catalogue No. G-1929-100
Description Cadherins are calcium-dependent cell adhesion proteins.
Batch No. See product label.
Unit size 100 ug
Antigen A synthetic peptide with sequence C-KQARAHGKSVPE, from the internal region of the protein sequence according to Q59EA3
Sequence KQARAHGKSVPE
Antigen Location Internal region
Antibody Type Polyclonal
Other Names CDH5; cadherin 5, type 2 (vascular endothelium); 7B4; CD144; 7B4 antigen; VE-cadherin; cadherin 5, type 2, VE-cadherin (vascular epithelium); cadherin-5; cd144 antigen; endothelial-specific cadherin; vascular endothelial cadherin
Accession Q59EA3(Q59EA3_HUMAN)
Produced in Goat
Purity Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Applications Peptide ELISA (1:128,000), Immunocytochemistry (10 ug/mL), Flow Cytometry (10 ug/mL). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Species Against Human
Cross-reactivity Pig and Cow
Form Liquid antibody. Supplied at 0.5 mg/mL in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin.
Storage Upon receipt, aliquot and store at -20C long-term. Store at 2-8C short-term (up to 2 weeks). Minimize freezing and thawing.
Expiry Date 12 months after purchase if unopened.
Images (click to zoom)
Goat antibody to Cadherin 5, type 2 preprotein variant (649-660): Affinity purified A. Immunofluorescence analysis: of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL). B. Flow cytometry analysis: of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

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MSDS

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