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Goat antibody to Mast stem cell growth factor receptor KIT (CD117, 685-697): Affinity purified

$347.00USD


Catalogue No. G-1927-100
Description Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine KITLG/SCF and plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis.
Batch No. See product label.
Unit size 100 ug
Antigen A synthetic peptide with sequence KRDSFICSKQEDH, from the internal region of the protein sequence according to P10721
Sequence KRDSFICSKQEDH
Antigen Location Internal region
Antibody Type Polyclonal
Other Names KIT; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog; C-Kit; CD117; PBT; SCFR; mast/stem cell growth factor receptor Kit; p145 c-kit; piebald trait protein; proto-oncogene c-Kit; proto-oncogene tyrosine-protein kinase Kit; soluble KIT varian
Accession P10721(KIT_HUMAN)
Produced in Goat
Purity Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Applications Peptide ELISA (1:128000), Western Blot (1-3 ug/mL), Immunocytochemistry (10 ug/mL), Flow Cytometry (10 ug/mL). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Specificity Approx. 110 kDa band observed in Human Brain (Hippocampus) lysates (calculated MW of 110 kDa according to P10721). An additional band of unknown identity was also consistently observed at 35 kDa. This band was successfully blocked by incubation with the immunizing peptide.
Species Against Human
Cross-reactivity Dog, Pig and Cow
Form Liquid antibody. Supplied at 0.5 mg/mL in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin.
Storage Upon receipt, aliquot and store at -20C long-term. Store at 2-8C short-term (up to 2 weeks). Minimize freezing and thawing.
Expiry Date 12 months after purchase if unopened.
Images (click to zoom)
Goat antibody to Mast stem cell growth factor receptor KIT (CD117, 685-697): Affinity purified A. Western Blot: (1 ug/mL) staining of Human Hippocampus lysate (A) + peptide (B) (35 ug protein in RIPA buffer). Detected by chemiluminescence. B. Flow cytometry analysis: of paraformaldehyde fixed MCF7 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Goat antibody to Mast stem cell growth factor receptor KIT (CD117, 685-697): Affinity purified A. Immunofluorescence analysis: of paraformaldehyde fixed HEK293 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic/membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml). B. Immunofluorescence analysis: of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic/membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
PDF Data Sheet

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MSDS

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