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Goat antibody to Prominin-1 (PROM1, CD133, 310-322): Affinity purified

$347.00USD


Catalogue No. G-1926-100
Description May play a role in cell differentiation, proliferation and apoptosis (PubMed:24556617). Binds cholesterol in cholesterol-containing plasma membrane microdomains and may play a role in the organization of the apical plasma membrane in epithelial cells. During early retinal development acts as a key regulator of disk morphogenesis.
Batch No. See product label.
Unit size 100 ug
Antigen A synthetic peptide with sequence HPSSETCNSIRLS, from the internal region of the protein sequence according to O43490
Sequence HPSSETCNSIRLS
Antigen Location Internal region
Antibody Type Polyclonal
Other Names PROM1; prominin 1; AC133; CD133; CORD12; MCDR2; PROML1; RP41; STGD4; OTTHUMP00000217744; OTTHUMP00000217745; OTTHUMP00000217746; antigen AC133; hProminin; hematopoietic stem cell antigen; prominin-1; prominin-like 1; prominin-like protein 1
Accession O4349O(PROM1_HUMAN)
Produced in Goat
Purity Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Applications Peptide ELISA (1:32,000), Immunocytochemistry (10 ug/mL), Flow Cytometry (10 ug/mL). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Species Against Human
Form Liquid antibody. Supplied at 0.5 mg/mL in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin.
Storage Upon receipt, aliquot and store at -20C long-term. Store at 2-8C short-term (up to 2 weeks). Minimize freezing and thawing.
Expiry Date 12 months after purchase if unopened.
Images (click to zoom)
Goat antibody to Prominin-1 (PROM1, CD133, 310-322): Affinity purified A. Immunofluorescence analysis: of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic/membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL). B. Immunofluorescence analysis: of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
Goat antibody to Prominin-1 (PROM1, CD133, 310-322): Affinity purified Flow cytometric analysis: of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation overnight (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
PDF Data Sheet

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MSDS

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