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Mouse anti-Microtubule Associated Protein 2 (MAP2) [5H11]: IgG


Catalogue No. M-1625-100
Description Microtubules are 25nm diameter protein rods found in most kinds of eukaryotic cells. They are polymerized from a dimeric subunit made of one 'a' subunit and one 'b' tubulin subunit. Microtubules are associated with a family of proteins called microtubule associated proteins (MAPs), which includes the protein t (tau) and a group of proteins referred to as MAP1, MAP2, MAP3, MAP4 and MAP5. MAP2 is made up of two ~280kDa apparent molecular weight bands referred to as MAP2 a and MAP2 b. A third lower molecular weight form, usually called MAP2c, corresponds to a pair of protein bands running at ~70kDa on SDS-PAGE gels. All these MAP2 forms are derived from a single gene by alternate transcription, and all share a C-terminal sequence which includes either three or four microtubule binding peptide sequences, which are very similar to those found in the related microtubule binding protein t (tau). MAP2 isoforms are expressed only in neuronal cells and specifically in the perikarya and dendrites of these cells. Antibodies to MAP2 are therefore excellent markers on neuronal cells, their perikarya and neuronal dendrites.
Batch No. See product label
Unit size 100 uL
Antigen High molecular MAP protein preparation derived from bovine brain
Clone 5H11
Other Names Microtubule-associated protein 2; MAP-2; Mtap2;
Accession Q0IIA8 MAP2_Bovine
Produced in Mouse
Molecular Weight http://
Purity IgG
Applications Immunohistochemistry (IHC), Immunocytochemistry (ICC) and Western Blotting (WB). A dilution of 1:1,000 - 1:5,000 is recommended for IHC and ICC, and 1:5,000-1:10,000 is recommended for WB. The optimal dilution should be determined by the end user.
Specificity The specificity of this antibody has been confirmed by WB and IHC against the antigen.
Cross-reactivity Human; Rat; Mouse;
Blast it If you wish to use this product in a species other than those specified here and want to determine the amino acid sequence homology between the immunogen and the corresponding protein in that species, simply copy the amino acid sequence for this immunogen and test it on the BLAST database that you can access through the link below. Please note that antibodies raised against synthetic peptides are quite often very specific for that peptide. What this means in practise is that a single amino acid difference may be enough to restrict the specificity to a particular molecule. The BLAST database can also be used to compare any amino acid sequence between species and between protein. The full amino acid sequence of the target protein can be found by following the link in the Accession field. You can then search any amino acid sequence in the target protein using the BLAST database. When selecting a sequence to search, please choose carefully, as the sequence you choose may be a precursor rather than the mature protein for example.
Form Liquid in PBS with 50% glycerol and 5mM NaN3.
Storage Maintain unopened vial at -20degC for up to 12 months after purchase. After opening, aliquot and store at -20C for a higher stability for up to six months. Avoid freeze-thaw cycles.
Expiry Date 12 months after purchase.
Images (click to zoom)
Mouse anti-Microtubule Associated Protein 2 (MAP2) [5H11]: IgG Whole rat brain lysate with mouse anti-MAP2 antibody. The antibody recognizes the ~280 kDa protein. Right: Mixed neuron/glia cultures stained with mouse anti-MAP2 (green) and also rabbit antibody to neurofilament H (Catalog Number R-1388-50) (red). Since the NF-H protein is largely expressed in neuronal axons, while the MAP2 is only found in neuronal dendrites and perikarya, there is little overlap between these two staining patterns. DNA stain shows nuclei of neurons and non-neuronal cells (blue).
Mouse anti-Microtubule Associated Protein 2 (MAP2) [5H11]: IgG Left: Rat hippocampus section stained by Immunohistochemistry with mouse anti-MAP2 (green, 1:5,000) and rabbit antibody to alpha-internexin (R-1379-50, red, 1:2,000). IHC Method: Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post-fixed for 24 hours, cut to 45 um, and free-floating sections were stained. MAP2 protein is detected in the perikarya and dendrites of the most neurons, and the alpha-internexin antibody selectively stains axons and dendrites of neuronal cells. Right: Western blot analysis of tissue lysates using mouse anti-MAP2 (green, 1:10,000). [1] protein standard, [2] adult rat whole brain, [2] embryonic (E20) rat brain, [4] adult rat spinal cord, and [5] adult mouse brain. A band at about 280 kDa corresponds to full length MAP2A and MAP2B protein. MAP2A/B is expressed heavily in adult brain particularly in cortical regions, but is a more minor component of spinal cord and almost absent from the embryonic brain sample. Note that the epitope for this antibody in within the projection domain found only in MAP2A and MAP2B, thus the antibody does not bind to the lower molecular weight MAP2C and MAP2D isoforms which lack this region.
Mouse anti-Microtubule Associated Protein 2 (MAP2) [5H11]: IgG MAP2 staining in fixed and permeabilized striatal neurons (7 DIV) by Immunocytochemistry. Primary antibody concentration: 1 ug/mL. Image courtesy of QBM Cell Science.
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