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Mouse antibody to Glial Fibrillary Acidic Protein (GFAP) [2A5]: affinity purified


Catalogue No. M-1827-100
Description GFAP is a 50 kDa intra-cytoplasmic filamentous protein of the cytoskeleton in astrocytes. During the development of the central nervous system, it is a cell-specific marker that distinguishes astrocytes from other glial cells. GFAP immunoreactivity has been shown in immature oligodendrocytes, epiglottic cartilage, pituicytes, papillary meningiomas, myoepithelial cells of the breast and in non-CNS: Schwann cells, salivary gland neoplasms, enteric glia cells, and metastasizing renal carcinomas.
Batch No. See product label
Unit size 100 ug
Antigen GFAP isolated biochemically from pig spinal cord was used as the immunogen.
Antigen Location The epitope for this antibody has been mapped to the N-terminal region of the alpha-helical coiled-coil rod-region of human GFAP isotype I, specifically within amino acids 71-217.
Antibody Type Monoclonal
Isotype IgG1
Clone 2A5
Other Names Glial fibrillary acidic protein; GFAP
Accession P14136 GFAP_HUMAN
Produced in Mouse
Purity Affinity purified
Applications Western Blot (1:1,000-1:2,000): tested on rat, mouse brain and spinal cord, human recombinant protein, pig brain. Immunohistochemistry (1:500-1:1,000): tested on rat cerebellum section. Other applications not yet tested. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Specificity This antibody is specific for GFAP as demonstrated by western blotting and immunohistochemistry.
Species Against Pig
Antibody Against GFAP
Cross-reactivity Pig, human, mouse, rat, cow
Form Lyophilized from a solution containing PBS pH 7.4 and 0.1% trehalose, containing 5 mM NaN3 as preservative.
Reconstitution Spin vial briefly before opening. Reconstitute in 100 uL sterile, ultrapure water to prepare 1 mg/mL solution. Centrifuge to remove any insoluble material.
Storage Store lyophilised antibody at 2-8C. After reconstitution divide into aliquots and store at -20C for long-term storage. Store at 2-8C short-term (up to 4 weeks) with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles.
Expiry Date 12 months after purchase if unopened.
Images (click to zoom)
Mouse antibody to Glial Fibrillary Acidic Protein (GFAP) [2A5]: affinity purified Left: Western blot analysis of GFAP expression in tissue homogenates. [1] protein standard, [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, [6] pig brain, [7] rat recombinant GFAP, [8] human recombinant GFAP. Bands around 50 kDa correspond to alternative transcripts and proteolytic products of GFAP. Primary antibody was diluted 1:2,000. Note that this antibody has significantly stronger reactivity with pig and human GFAP as compared to rodent, suggesting that it binds to an epitope which is not totally conserved across mammalian sequences. Right: Analysis of GFAP expression in an adult rat cerebellum section by Immunohistochemistry. Primary antibodies: mouse anti-GFAP (red, 1:500), chicken anti-Parvalbumin (C-1814-50, green, 1:2,000). Blue: DAPI nuclear stain. IHC Method: Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post-fixed for 24 hours, and free-floating 45 uM sections were stained. The GFAP antibody stains the processes of Bergmann glia and astrocytes. The Parvalbumin antibody labels perikarya and dendrites of Purkinje cells and interneurons in the molecular layer of the cerebellum. The staining on rodent tissues is specific but not as robust as on human material.

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