biosensis fine bioscience tools - antibodies, elisa kits, proteins, toxins and peptides An Australia-US partnershipPhone: 1800 605 5127 in the US, or +61 8 8352 7711 for international enquiries to Australia.
HomeAbout BiosensisBiosensis distributorsResourcesHelp deskordering from BiosensisContact usFlyers

Technical Notes
View our tech notes
Follow us...
Google+ Follow us on Twitter

Find us on Facebook

Sign up for Biosensis newsletters
Visit our newsletter archive

Mouse antibody to human Ki-67 [6B4]: affinity purified


Catalogue No. M-1805-100
Description Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly (PubMed:27362226). Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface (PubMed:27362226). Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility (PubMed:27362226). Binds DNA, with a preference for supercoiled DNA and AT-rich DNA (PubMed:10878551). Does not contribute to the internal structure of mitotic chromosomes (By similarity). May play a role in chromatin organization (PubMed:24867636). It is however unclear whether it plays a direct role in chromatin organization or whether it is an indirect consequence of its function in maintaining mitotic chromosomes dispersed (Probable). Ref:
Batch No. See product label.
Unit size 100 ug
Antigen Recombinant human Ki-67 protein (amino acids 1,111-1,490) expressed in and purified from E. coli.
Isotype IgG1
Clone 6B4
Other Names Proliferation marker protein Ki-67; Antigen identified by monoclonal antibody Ki-67; Antigen KI-67; Antigen Ki67
Accession P46013 (KI67_HUMAN)
Produced in Mouse
Purity Affinity purified
Applications Western blotting (1:1,000-1:5,000) and Immunocytochemistry (1:2,000-1:5,000). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Specificity Reacts with human only. Does not react with mouse or rat.
Form Lyophilised from a solution containing PBS buffer pH 7.2-7.6 with 5 mM sodium azide as preservative.
Reconstitution Spin vial briefly before opening. Reconstitute in 100 uL sterile water. Centrifuge to remove any insoluble material. Final buffer contains preservatives.
Storage Store lyophilised antibody at 2-8C. After reconstitution divide into aliquots and store at -20C for long-term storage. Store at 2-8C short-term (up to 4 weeks). Avoid repetitive freeze/thaw cycles.
Expiry Date 12 months after purchase if unopened.
Images (click to zoom)
Mouse antibody to human Ki-67 [6B4]: affinity purified Left: Immunofluorescence analysis of HeLa cells costained with mouse antibody to Ki-67 (1:2,000, red) and chicken antibody to vimentin (C-1409-50, 1:10,000, green). Ki-67 protein is predominantly localized in nucleoli of _cells in interphase, while in mitotic cells Ki-67 is associated with condensed chromosome. Ki-67 is not expressed in cells in quiescent G0 state. Blue: DAPI staining of nuclear DNA. Right: Western blot analysis of equal amounts of HeLa (Lane 2) and HEK293 (Lane 3) cell lysates probed with mouse antibody to Ki-67 (1:2,000, Lanes 2 and 3). Two bands above 250 kDa are observed, corresponding to major Ki-67 isoforms of molecular weight ~345 and 395 kDa.
Mouse antibody to human Ki-67 [6B4]: affinity purified Analysis of Ki67 expression (green) in cultured HeLa cells with mouse anti-Ki67 antibody by Immunocytochemistry. The nuclei of rapidly dividing cells have clumps of Ki67 associated with nucleoli, while nearby quiescent cells express little or no Ki67. Cells are counterstained with chicken anti-lamin A/C antibody (C-1698-100, red) which reveals the nuclear lamina. Blue: DAPI nuclear stain.
PDF Data Sheet

Click to download the data sheet - Click to download the Data Sheet


Click to download the MSDS - Click to download the MSDS

Offices in the USA and Australia
My Ice Bucket  
0 items
Email This Page
Tell someone you know about this product.
We Accept PayPal
You can use secured services of paypal to send us money via our email account MODULE_PAYMENT_PAYPAL_IPN_IDYou can use secured services of paypal to send us money via our email account MODULE_PAYMENT_PAYPAL_IPN_IDYou can use secured services of paypal to send us money via our email account MODULE_PAYMENT_PAYPAL_IPN_ID
Click to make a payment

Official PayPal Seal