| Catalogue No. |
M-996-100 |
| Description |
Actins are highly conserved proteins ubiquitously expressed in all eukaryotic cells. Three main groups of actin isoforms have been identified in vertebrates; alpha, beta and gamma. Alpha actins are found mainly in muscle tissues. |
| Batch No. |
See product label |
| Unit size |
100 ug |
| Antigen |
A synthetic peptide of 10 amino acids corresponding to the N-terminus of alpha smooth muscle actin. This antibody reacts only with alpha smooth muscle actin and does not react with actin from striated muscle (alpha-sarcomeric) and myocardium (alpha-myocardial). It also does not react with beta and gamma actins. |
| Clone |
1A4 |
| Other Names |
ACTA2; ACTSA; ACTVS; |
| Accession |
P62736 ACTA_HUMAN |
| Produced in |
Mouse |
| Purity |
IgG2a |
| Applications |
Immunohistochemistry (IHC) and Western Blotting (WB). A concentration of 2.0 ug/mL is recommended for WB. Human smooth muscle actin has a predicted length of 377 residues and a MW of 42 kDa. A concentration of 0.1-0.5 ug/mL is recommended to detect alpha smooth muscle actin in formalin fixed and paraffin embedded tissues (heat retrieval is recommended) as well as formalin/acetone fixed tissues. Biosensis recommends optimal dilutions/concentrations should be determined by the end user. |
| Specificity |
The specificity of this antibody has been confirmed by WB and IHC against the antigen. |
| Cross-reactivity |
Human; mouse; rat; |
| Blast URL |
Click here |
| Form |
Lyophilized from 1.2% sodium acetate, 2mg BSA, 0.01mg NaN3 |
| Reconstitution |
Reconstitute in 1 mL of PBS to achieve an antibody concentration of 100 ug/mL. Centrifuge to remove any insoluble material. |
| Storage |
At least 12 months after purchase at 2-8C (lyophilized formulations). After reconstitution, aliquot and store at -20C for up to six months. Avoid freeze-thaw cycles. |
| Expiry Date |
12 months after purchase, unopened. |
| Specific References |
1. Pogribny IP et al (2013) Strain-dependent dysregulation of one-carbon metabolism in male mice is associated with choline- and folate-deficient diet-induced liver injury.
FASEB J. 2013 Jun;27(6):2233-43.
2. Sebastiani G et al (2011) Accelerated CCl4-induced liver fibrosis in Hjv-/- mice, associated with an oxidative burst and precocious profibrogenic gene expression.
PLoS One. 2011;6(9):e25138.
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