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Mouse monoclonal antibody to TAR DNA-binding protein 43 (TDP43), [3H8]

$297.00USD


Catalogue No. M-1403-100
Description TAR DNA-binding protein 43 (TDP43) is a DNA and RNA-binding protein which regulates transcription and splicing (ref: SWISSPROT).
Batch No. See product label
Unit size 100 uL
Antigen This antibody was raised against recombinant full length human his-tagged TDP43 which was expressed in E. coli and purified by nickel affinity.
Antibody Type Monoclonal
Isotype IgG
Clone 3H8
Other Names TAR DNA-binding protein 43; TDP-43; TARDBP; TDP43;
Accession Q13148 TADBP_HUMAN;
Produced in Mouse
Applications Western Blotting (WB), Immunocytochemistry (ICC) and Immunohistochemistry (IHC). A dilution of 1:1,000 - 1:5,000 is recommended for WB and IHC. A dilution of 1:500-1,000 is recommended for IC. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Specificity The specificity of this antibody has been confirmed by WB. This antibody detects ~43 kDa TDP43 protein on crude extract of mouse brain nuclear fraction.
Antibody Against TAR DNA-binding protein 43
Cross-reactivity Human and Rodent. Predicted to react with other mammalian tissue.
Blast URL Click here
Form Lyophilised with 5% trehalose
Appearance White powder
Reconstitution Reconstitute in sterile distilled water. Centrifuge to remove any insoluble material.
Storage After reconstitution of lyophilised antibody, aliquot and store at -20C for a higher stability. Avoid freeze-thaw cycles.
Expiry Date 12 months after purchase
Images (click to zoom)
Mouse monoclonal antibody to TAR DNA-binding protein 43 (TDP43), [3H8] Mouse monoclonal antibody to TAR DNA-binding protein 43 [3H8] M-1403-100 was used to stain a section of formalin fixed adult rat brain, specifically the hippocampus. Hippocampal neuron nuclei are stained strongly. Chicken polyclonal antibody to GFAP C-1373-50 (green) shows the processes of astrocytic glial cells. Nuclei of all cells are revealed with DAPI DNA stain (blue). The TAR DNA-binding protein 43 antibody stains neuronal nuclei strongly and the nuclei of some non-neuronal cells much more weakly. Neuronal nuclei therefore look crimson, since they are both red due to the content of TAR DNA-binding protein 43 and blue due to their content of DNA, stained blue with DAPI.
Mouse monoclonal antibody to TAR DNA-binding protein 43 (TDP43), [3H8] Left: Analysis of TDP43 expression in rat hippocampus section by Immunohistochemistry. Section was stained with mouse anti-TDP43 antibody (red, 1:2,000), and co-stained with chicken antibody to GFAP (C-1373-50, green, 1:5,000). Blue: DAPI nuclear stain. IHC method: Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post-fixed for 24 hours, cut to 45 um sections, and free-floating sections were stained. The TDP43 protein is concentrated in the nuclei of hippocampal neurons, while the GFAP antibody stains the intermediate filament network of astroglial cells. Right: Western blot analysis of whole brain lysates and nuclear extracts for TDP43 expression (green, 1:2,000). [1] protein standard, [2] rat brain, [3] rat brain nuclear extract, [4] mouse brain, [5] mouse brain nuclear extract. A strong band at 43 kDa corresponds to TDP43 protein.
Mouse monoclonal antibody to TAR DNA-binding protein 43 (TDP43), [3H8] TDP43 staining in fixed and permeabilized human umbilical vein endothelial cells (HUVEC) by Immunocytochemistry. TDP43-IR (green) is localized to cell nuclei as expected. Blue: Hoechst nuclear stain (left only). Red: Phalloidin staining. Primary TDP43 antibody concentration: 2 ug/mL; Magnification: 32X. Image courtesy of QBM Cell Science.
Mouse monoclonal antibody to TAR DNA-binding protein 43 (TDP43), [3H8] TDP43 staining in fixed and permeabilized human umbilical vein endothelial cells (HUVEC) by Immunocytochemistry. TDP43-IR (green) is localized to cell nuclei as expected. Red: Staining of von Willebrandt factor. Primary TDP43 antibody concentration: 2 ug/mL; Magnification: 32X. Image courtesy of QBM Cell Science.
Mouse monoclonal antibody to TAR DNA-binding protein 43 (TDP43), [3H8] Staining of fixed and permeabilized rat hippocampal cells for TDP43 (green) and NeuN (red) by Immunocytochemistry. Green: Mouse antibody to TDP43, M-1403-100 (2 ug/mL); Red: Rabbit antibody to NeuN, R-3770-100 (1:500 dilution); Blue: Hoechst nuclear dye. TDP43-IR and NeuN-IR is localized to cell nuclei as expected. Magnification: 32X. Image courtesy of QBM Cell Science.
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