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Mouse monoclonal antibody to pan Cadherin [CH-19]: IgG


Catalogue No. M-1740-100
Description Cadherins are transmembrane glycoproteins that mediate calcium ion-dependent cell adhesion. The cadherin family includes N-, P-, R-, B- and E-cadherins which are found in adherens junctions.
Batch No. See product label
Unit size 100 ug
Antigen A synthetic peptide corresponding to the C-terminal amino acids of chicken N-Cadherin with an extra N-terminal lysine residue (24 amino acids) coupled to KLH.
Other Names CDH; N-cadherin; Neural Cadherin; Cadherin-2; P-cadherin; R-cadherin; B-cadherin; E-cadherin;
Accession P19022 CADH2_HUMAN; P10288 CADH2_CHICK;
Produced in Mouse
Purity IgG
Applications Immunohistochemistry (IHC) and Western Blotting (WB). A concentration of 1.0-2.0 ug/mL is recommended for WB. Human N-Cadherin has a predicted length of 906 residues and MW of 100 kDa. A concentration of 2.0-4.0 ug/mL is recommended for IHC to detect the protein in paraffin embedded tissues. Antigen retrieval by heat is required. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Specificity The specificity of this antibody has been confirmed by WB and IHC against the antigen.
Cross-reactivity Human; mouse; rat; rabbit; chicken; snake;
Blast it If you wish to use this product in a species other than those specified here and want to determine the amino acid sequence homology between the immunogen and the corresponding protein in that species, simply copy the amino acid sequence for this immunogen and test it on the BLAST database. Please note that antibodies raised against synthetic peptides are quite often very specific for that peptide. What this means in practise is that a single amino acid difference may be enough to restrict the specificity to a particular molecule. The BLAST database can also be used to compare any amino acid sequence between species and between protein. The full amino acid sequence of the target protein can be found by following the link in the Accession field. You can then search any amino acid sequence in the target protein using the BLAST database. When selecting a sequence to search, please choose carefully, as the sequence you choose may be a precursor rather than the mature protein for example.
Form Lyophilized. Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
Reconstitution Reconstitute in 1 mL of PBS to achieve an antibody concentration of 100 ug/mL. Centrifuge to remove any insoluble material.
Storage At -20C for one year. After reconstitution, at 2-8C for one month. It can also be aliquotted and stored frozen at -20C for a longer time. Avoid repeated freezing and thawing.
Expiry Date 12 months after purchase.
Specific References Kawai T and Shimada M (2020). Pretreatment of ovaries with collagenase before vitrification keeps the ovarian reserve by maintaining cell-cell adhesion integrity in ovarian follicles. Sci Rep. 10(1):6841; Application: IHC/IF, WB Species: Mouse
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