||Calponin is a thin filament associated protein that binds calmodulin, actin and tropomyosin. The multi-gene family of calponins includes calponin 1 (CNN1), 2 (CNN2) and 3 (CNN3). CNN1 is expressed in smooth muscle and tissues containing significant amounts of smooth muscle. This antibody is predicted to detect all three forms of calponin.
||See product label
||Turkey gizzard calponin
||CNN1; Calponin-1; CNN2; CNN3;
|| P51911 CNN1_HUMAN;
||Immunohistochemistry (IHC) , Immunocytochemistry (IC) and Western Blotting (WB). A concentration of 0.25-0.50 ug/mL is recommended for WB. Human calponin has a predicted length of 297 aa and a MW of 34 kDa. A concentration of 0.5-1.0 ug/mL is recommended to detect calponin in formalin fixed and paraffin embedded tissues. Heat retrieval is recommended. A concentration of 1 ug/mL is recommended for immunocytochemistry. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
||The specificity of this antibody has been confirmed by WB and IHC against the antigen.
||Human; chicken; turkey
||If you wish to use this product in a species other than those specified here and want to determine the amino acid sequence homology between the immunogen and the corresponding protein in that species, simply copy the amino acid sequence for this immunogen and test it on the BLAST database that you can access through the link below. Please note that antibodies raised against synthetic peptides are quite often very specific for that peptide. What this means in practise is that a single amino acid difference may be enough to restrict the specificity to a particular molecule.
The BLAST database can also be used to compare any amino acid sequence between species and between protein. The full amino acid sequence of the target protein can be found by following the link in the Accession field. You can then search any amino acid sequence in the target protein using the BLAST database. When selecting a sequence to search, please choose carefully, as the sequence you choose may be a precursor rather than the mature protein for example.
||Lyophilized from 1.2% sodium acetate, 2mg BSA, 0.01mg NaN3
||At least 12 months after purchase at 2-8C (lyophilized formulations). After reconstitution, aliquot and store at -20C for a higher stability. Avoid freeze-thaw cycles.
||12 months after purchase (unopended).