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Mouse monoclonal antibody to rat capsaicin receptor (819-838) [5G8A9G8]: IgG


Catalogue No. M-008-100
This product is no longer available. Please see below for our recommended alternative products.
Replaced by M-1714-100
Batch No. See product label
Unit size 100 g
Antigen A synthetic peptide (C-GSLKPEDAEVFKDSMVPGEK) as a part of rat VR1 protein (aa: 819-838) conjugated to KLH has been used as the immunogen.
Clone 5G8A9G8
Other Names VR1; Transient receptor potential cation channel subfamily V member 1; TrpV1; osm-9-like TRP channel 1; OTRPC1; Vanilloid receptor 1; Capsaicin receptor; VR-1
Accession TRPV1_RAT
Produced in Mouse
Purity Protein G purified immunoglobulin
Applications IHC. Recommended to be used at a concentration of 0.5-1 g/ml. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Specificity A high level of specificity has been confirmed by immunohistochemical staining of rat DRG.
Cross-reactivity This antiserum is known to react with rat and guinea pig. It is predicted to react with mouse due to sequence homology.
Blast it If you would like to use this product in another species other than those specified here, or to see the shared identity between the immunogen used here in different species and/or other molecules, simply copy the immunogen (from the Immunogen field) and paste it HERE and blast/format it. Note that, antisera raised against synthetic peptides are quite often very specific for that peptide ie only one single amino acid difference may be enough to restrict the specificity to a particular molecule.
Form Lyophilised
Reconstitution Reconstitute in 100 l of sterile water. Centrifuge to remove any insoluble material.
Storage After reconstitution keep aliquots at -20C for a higher stability and at 4C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Expiry Date 12 months after purchase.
Specific References 1. Peng H.Y. (2008) TRPV1 mediates the uterine capsaicin-induced NMDA NR2B-dependent cross-organ reflex sensitization in anesthetized rats. Am J Physiol Renal Physiol. Nov;295(5):F1324-35.
Images (click to zoom)
Mouse monoclonal antibody to rat capsaicin receptor (819-838) [5G8A9G8]: IgG Immunohistochemical detection of capsaicin receptor in rat dorsal root ganglia using Mouse monoclonal antibody to rat capsaicin receptor (819-838): IgG, catalogue number M-008-100, Clone number 5G8A9G8. Anaesthetised rat was perfused with 4% paraformaldehyde and dorsal root ganglia were postfixed in the same fixatives for 2 hours. The cryostat sections were stained with Monoclonal antibody to capsaicin receptor at the concentration of 0.5 µg/ml and developed with a DAB method with nickel enhancement. The photo taken at 20x magnification. A subpopulation of sensory neurons were stained.

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