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Mouse Monoclonal Antibody to Galectin-3 [5C21]: Affinity purified


Catalogue No. M-1574-100
Description Galectin 3 is a lectin with carbohydrate recognition domains (CRD) which bind -galactoside. It is a multifunctional protein expressed both on the cell surface, cytoplasm and nucleus and appears to have roles in specific carbohydrate binding and in the regulation of mRNA splicing.
Batch No. See product label
Unit size 100 ug
Antigen Full length recombinant Galectin-3 expressed in and purified from E. coli.
Antibody Type monoclonal
Isotype IgG1
Clone 5C21
Other Names Macrophage galactose-specific lectin; MAC2; galactoside-binding protein; GALBP; Galectin 3; Gal 3; 35 kDa lectin; Carbohydrate-binding protein 35; CBP 35; Galactose-specific lectin 3; IgE-binding protein; L-31; Laminin-binding protein; Lectin L-29; Mac-2 antigen; LGALS3;
Accession P17931 LEG3_HUMAN;
Produced in Mouse
Applications WB, ICC. Suggested dilution of at least 1:1,000 for ICC. Suggested dilution of 1:2,000 or lower for WB. Optimal concentrations/dilutions should be determined by the end-user.
Cross-reactivity Human; Mouse; Rat; predicted to react with other species due to sequence homology;
Blast URL Click here
Form Lyophilised with 5% trehalose
Reconstitution Reconstitute in sterile distilled water. Centrifuge to remove any insoluble material.
Storage After reconstitution of lyophilised antibody, aliquot and store at -20C for a higher stability. Avoid freeze-thaw cycles.
Expiry Date 12 months after purchase
Images (click to zoom)
Mouse Monoclonal Antibody to Galectin-3 [5C21]: Affinity purified Image shows rat brain neural cultures stained with Mouse Monoclonal Antibody to Galectin-3 M-1574-100 (green) and DNA (blue). Staining can be seen in several types of glia and lymphocytic cells, including these cells which have the morphology of microglia. Surrounding cells reveal no Galectin-3 staining.
Mouse Monoclonal Antibody to Galectin-3 [5C21]: Affinity purified Left: Analysis of galectin-3 expression in cortical neuron-glial cell culture from E20 rat by Immunocytochemistry. Cells were stained with mouse antibody to galectin-3 (red, 1:1,000), and co-stained with chicken antibody to MAP2 (C-1382-50, green, 1:10,000). Blue: Hoechst nuclear stain. The galectin-3 antibody produces strong staining of microglia (top panel), other glial cells (bottom panel) and lymphocytic cells (small round cells), while MAP2-antibody labels dendrites and perikarya of mature neurons. Right: Western blot analysis of tissue and cell lysates using mouse antibody to galectin-3 (green, 1:2,000): [1] protein standard, [2] mouse brain lysate; [3] [7] rat tissue lysates: [3] brain, [4] liver, [5] heart, [6] kidney, [7] lung, and [8] HEK293 cell lysate. The band at 30 kDa mark corresponds to the galectin-3 protein. The same blot was simultaneously probed with an antibody to HSP60 (red, lanes 2-8).
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