Mouse monoclonal antibody to Glial Fibrillary Acidic Protein (GFAP) [5C10]: IgG

$297.00USD

Catalogue No.	M-1375-100
Description	GFAP is a 50 kDa intra-cytoplasmic filamentous protein of the cytoskeleton in astrocytes. During the development of the central nervous system, it is a cell-specific marker that distinguishes astrocytes from other glial cells. GFAP immunoreactivity has been shown in immature oligodendrocytes, epiglottic cartilage, pituicytes, papillary meningiomas, myoepithelial cells of the breast and in non-CNS: Schwann cells, salivary gland neoplasms, enteric glia cells, and metastasizing renal carcinomas.
Batch No.	See product label
Unit size	100 uL
Antigen	Purified GFAP from porcine spinal cord
Antibody Type	Monoclonal
Isotype	IgG1
Clone	5C10
Other Names	Astrocyte; Glial fibrillary acidic protein; GFAP;
Accession	P14136 GFAP_HUMAN; Q8WP16 Q8WP16_PIG;
Produced in	Mouse
Applications	Western Blotting (WB), Immunocytochemistry (ICC) and Immunohistochemistry (IHC). A dilution of 1:5,000 is recommended for WB. Human GFAP has a predicted length of 432 residues and a MW of 50 kDa. A dilution of 1:500-1:1,000 is recommended for ICC/IHC. This antibody works well on frozen sections, cells in tissue culture and on formalin fixed histological sections. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Specificity	The specificity of this antibody has been confirmed by WB.
Antibody Against	Glial Fibrillary Acidic Protein
Cross-reactivity	Human, Rat, Mouse, Bovine, Porcine. Predicted to react with other mammalian and avian species.
Blast URL	Click here
Form	Lyophilised from PBS, pH 7.4, containing 3% trehalose and 0.05% sodium azide.
Reconstitution	Reconstitute in 100 uL sterile distilled water to obtain an antibody stock concentration of 1 mg/mL. Centrifuge to remove any insoluble material.
Storage	After reconstitution of lyophilised antibody, aliquot and store at -20C for a higher stability. Avoid freeze-thaw cycles.
Expiry Date	12 months after purchase if unopened
Specific References	Kawabe K et al. (2017) Transglutaminases Derived from Astrocytes Accelerate Amyloid β Aggregation. Neurochem Res. [Epub ahead of print]. Application: ICC (cultured rat astrocytes). Nagai T et al. (2017) Development of an in situ evaluation system for neural cells using extracellular matrix-modeled gel culture. J Biosci Bioeng. 124(4):430-8. Application: IF (artificial gel matrix). Kawabe T et al. (2017) Microglia Endocytose Amyloid β Through the Binding of Transglutaminase 2 and Milk Fat Globule EGF Factor 8 Protein. Neurochem Res. [Epub ahead of print] Application: ICC (cultured astrocytes). Takano K et al. (2017) Inhibition of Gap Junction Elevates Glutamate Uptake in Cultured Astrocytes. Neurochem Res. [Epub ahead of print] Application: ICC (cultured astrocytes).
References	1. Reeves S.A, et al. Proc. Natl. Acad. Sci. U.S.A. 86:5178-5182(1989). 2. Brenner M, et al. Brain Res. Mol. Brain Res. 7:277-286(1990). 2. Isaacs A, et al. Genomics 51:152-154(1998). 3. Ota T, et al. Nat. Genet. 36:40-45(2004). 4. Nielsen A.L, et al. J. Biol. Chem. 277:29983-29991(2002). 5. Singh R, et al. Genomics 82:185-193(2003). 6. Brenner M, et al. Nat. Genet. 27:117-120(2001). 7. Brockmann K, et al. Eur. Neurol. 50:100-105(2003). 8. Stumpf E, et al. Arch. Neurol. 60:1307-1312(2003). 9. Sawaishi Y, et al. Neurology 58:1541-1543(2002). 10. Aoki Y, et al. Neurosci. Lett. 312:71-74(2001).

Images (click to zoom)

Mouse monoclonal antibody to Glial Fibrillary Acidic Protein (GFAP) [5C10]: IgG

Mixed neuron-glial cultures stained with Mouse monoclonal antibody to Glial Fibrillary Acidic Protein [5C10] M-1375-100 (red) and chicken polylclonal antibody to neurofilament L C-1390-50 (green). The GFAP antibody stains the network of astrocytes in these cultures, while the NF-L antibody stains neurons and their processes. The blue channel shows the localization of DNA.

Right: Detection of GFAP expression in rat cerebellum by Immunohistochemistry with mouse antibody to GFAP (1:1,000, green), and co-stained with rabbit antibody to NF-L (R-1392-50, 1:2,000, red). IHC Method: Following transcardial perfusion with 4% PFA, brain was post-fixed for 24 hours, cut to 45 um sections, and free-floating sections were stained. Clone 5C10 stains a network of astroglial cells, while the NF-L antibody labels neuronal cells and their processes. Right: Western blot analysis of whole tissue lysates using mouse antibody to GFAP (1:2,000, green). [1] protein standard , [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord. The strong band at about 50 kDa corresponds to the GFAP protein.

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