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Mouse monoclonal antibody to human ATG3 [1F7]: IgG


Catalogue No. M-908-100
Description Autophagy is a process of bulk degradation of cytoplasmic components by the lysosome or vacuole. ATG3 is an E2-like protein-conjugating enzyme for GABARAPL1, GABARAPL2 and MAPLC3 binding them to PE. ATG3 forms an E1-E2 complex with ATG7 and cross-talks with the ATG12 conjugation system. ATG3 is a cytoplasmic protein that is widely expressed with highest expression in heart, skeletal muscle, kidney , liver and placenta.
Batch No. See product label
Unit size 100 ug
Antigen Partial recombinant human ATG3 [1-100] with a GST tag.
Isotype IgG2b, kappa
Clone 1F7
Other Names Autophagy-related protein 3; APG3-like; hApg3; Protein PC3-96; ATG3; APG3; APG3L
Accession ATG3_HUMAN
Produced in Mouse
Purity Protein G purified immunoglobulin
Applications This antibody is recommended for WB, sandwich ELISA and Flow Cytometry (2ug/10^6 cells). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Specificity Specificity has been confirmed by WB and ELISA against the antigen.
Cross-reactivity Human. Other species have not been tested.
Blast it To see the shared identity between different species or other proteins, follow the link in the Accession field, select the sequence that you are interested in and copy and paste it HERE and blast/format it.
Form Lyophilised from PBS pH 7.2
Reconstitution Reconstitute in 100 uL of sterile water. Centrifuge to remove any insoluble material.
Storage After reconstitution keep aliquots at -20C for higher stability or at 2-8C with an appropriate antibacterial agent. Glycerol (1:1) may be added for additional stability. Avoid repetitive freeze/thaw cycles.
Expiry Date 12 months after purchase
Images (click to zoom)
Mouse monoclonal antibody to human ATG3 [1F7]: IgG A: Flow Cytometry analysis of ATG3 expression in SH-SY5Y cells. Fixing and permeabilization of cells: absolute methanol and 0.1% Tween-20 in PBS; Primary antibody: Mouse Monoclonal antibody to ATG3 (cat # M-908-100, 2 μg per ~106 cells) for 30 minutes at room temperature; Secondary antibody: Goat anti-mouse PE (1:100 dilution) with incubation for 20 minutes in dark at room temperature. Non-specific Control IgG, clone X63 (cat # M-1249-200) was used as negative control under same conditions. Data and results were generated using Orflo MoxiflowTM instrument and protocols. B: Western blot detection of ATG3 expression in Jurkat cell lysate. C: Sandwich ELISA using the anti-ATG3 monoclonal antibody as capture antibody. The detection limit for GST-tagged recombinant ATG3 is 1 ng/mL.

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