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Mouse monoclonal antibody to human CRIM1 [6E4]: IgG


Catalogue No. M-817-100
Description CRIM1 may participate in CNS development by interacting with growth factors involved in motor neuron differentiation and survival. It also has a potential role in capillary formation and maintenance during angiogenesis. It also affects BMP processing and delivery to the cell surface. CRIM1 interacts with BMP4 and BMP7. It localises to the cell membrane and is expressed in a number of tissues including the pancreas, kidney, skeletal muscle, lung, placenta, brain, heart, spleen, liver, small intestine and blood vessels. The unprocessed precursor has a length of 1036 amino acids and a predicted molecular weight of 113.7KDa. CRIM1 contains four antistatin-like domains, one IGFBP N-terminal domain and six VWFC domains. It is also N-glycosylated.
Batch No. See product label
Unit size 100 ug
Antigen A partial recombinant human CRIM1 protein (amino acids 36 to 145) with a GST tag.
Clone [6E4]
Other Names Cysteine-rich motor neuron 1 protein; cysteine-rich repeat-containing protein S52; CRIM1
Accession CRIM1_HUMAN
Produced in Mouse
Purity Protein G purified immunoglobulin
Applications This antibody is recommended for IHC at a dilution of 3 ug/mL. It is also recommended for sandwich ELISA with a detection limit for the recombinant GST tagged CRIM1 protein of 0.03 ng/mL. This antibody is useful for WB analysis. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Specificity The specificity of this antibody has been confirmed by WB and ELISA against the antigen.
Cross-reactivity No other species have been tested.
Blast it If you would like to see the shared identity between different species or other proteins, follow the link in the Accession field, select the sequence (make sure that you are selecting the sequence that you are interested in, as the sequence may be the precursor rather than the mature protein for example) and copy and paste it HERE and blast/format it.
Form Lyophilised from PBS pH 7.2
Reconstitution Reconstitute in 100 uL of sterile water. Centrifuge to remove any insoluble material.
Storage After reconstitution keep aliquots at -20C for higher stability or at 2-8C with an appropriate antibacterial agent. Glycerol (1:1) may be added for additional stability. Avoid repetitive freeze/thaw cycles.
Expiry Date 12 months after purchase
Images (click to zoom)
Mouse monoclonal antibody to human CRIM1 [6E4]: IgG Immunohistochemical detection of CRIM1 in formalin-fixed paraffin-embedded human lung tissue using mouse monoclonal antibody to human CRIM1 (M-817-100) at a concentration of 3µg/ml.

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