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Mouse monoclonal antibody to human dystrophin [MANDYS8]


Catalogue No. M-1833-100
Description Dystrophin is a large cytoskeletal protein (426 kDa) found at the inner surface of muscle fibres. Dystrophin is part of the dystrophin glycoprotein complex (DGC), which bridges the inner cytoskeleton (F actin) and the extracellular matrix. The monoclonal antibody clone MANDYS8 was developed in K.E.Daviesí laboratory (see Nguyen Thi Man et al. 1990). This mouse monoclonal specifically recognizes an epitope located on the rod domain of the human dystrophin molecule. The antibody reacts with dystrophin from human, mouse, rat, rabbit, and porcine, but not canine or frog origin. This epitope is present in normal muscle tissue and in nearly all Becker muscular dystrophies. It is absent in cases of Duchenne muscular dystrophies and in the dystrophic mouse (mdx). The epitope recognized by the antibody is sensitive to formalin fixation and paraffin embedding.
Batch No. See product label
Unit size 100 ug
Antigen Recombinant human dystrophin encoding amino-acids 816-1749.
Antibody Type Monoclonal
Isotype IgG2b
Clone 8H11/MANDYS8
Other Names DMD; Dystrophin-4
Accession P11532 DMD_HUMAN
Produced in Mouse
Molecular Weight Human dystrophin has a predicted length of 3,685 residues and molecular weight of 427 kDa.
Purity Ascites
Applications Immunohistochemistry (IHC) and Western Blotting (WB). A dilution of 1:400 - 1:1,600 is recommended for WB. A concentration of 1:400 - 1:6,000 (ABC detection) is recommended to detect dystrophin in fresh frozen, or acetone fixed, 2% PLP fixed sections. Immunolocalization: stains muscle membranes (no staining of Duchenne muscle membrane). Not recommended for paraffin-embedded tissues. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Specificity The specificity of this antibody has been confirmed by WB and IHC against human samples.
Species Against Human
Cross-reactivity Mouse, rat, chicken, rabbit. Does not cross-react with dystrophin of canine or Xenopus origin.
Form Lyophilized from mouse ascites fluid in 1.2% sodium acetate, 2 mg BSA, with 0.01 mg NaN3 as preservative.
Reconstitution Reconstitute in 1 mL of PBS (pH 7.2 - 7.6) to achieve an antibody concentration of 100 ug/mL. Centrifuge to remove any insoluble material.
Storage Store lyophilized antibody at 2-8C. After reconstitution divide into aliquots and store at -20C for up to six months. Store at 2-8C short-term (up to 4 weeks). Avoid repetitive freeze/thaw cycles.
Expiry Date 12 months after purchase (lyophilized).
General References Nguyen Thi Man, A.J. Cartwright, G.E. Morris, D. Love, J.F. Bloomfield and K.E. Davies. Monoclonal antibodies against defined regions of the muscular dystrophy protein, dystrophin. FEBS Letters, 262 (1990) 237-240.

Nguyen Thi Man and G.E. Morris. Use of epitope libraries to identify exon-specific monoclonal antibodies for characterization of altered dystrophins in muscular dystrophy. American J.Human Genetics, 52 (1993) 1057-1066.
Images (click to zoom)
Mouse monoclonal antibody to human dystrophin [MANDYS8] Detection of dystrophin in normal human muscle by Western Blotting (left) and Immunohistochemistry (right).

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