| Catalogue No. |
M-844-100 |
| Description |
LIMK-1 belongs to a small subfamily of LIM proteins with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. LIMK1 regulates actin filament dynamics. It phoshorylates and inactivates the actin binding/ depolymerising factor cofilin, consequently stabilising the actin cytoskeleton. It may also be involved in brain development. LIMK-1 localises to the cytoplasm and is highly expressed in both the adult and fetal nervous system. It is expressed to a lesser extent in heart and skeletal muscle. LIMK-1 hemizygosity is implicated in the impaired visuospatial constructive cognition of Williams syndrome.
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| Batch No. |
See product label |
| Unit size |
100 ug |
| Antigen |
Partial recombinant protein of human LIMK-1 (aa 548 to 648) with a GST tag. |
| Clone |
1A8 |
| Other Names |
LIM domain kinase 1; LIMK1; LIMK |
| Accession |
LIMK1_HUMAN |
| Produced in |
Mouse |
| Purity |
Protein G purified immunoglobulin |
| Applications |
This antibody is recommended for WB, IHC and sandwich ELISA. For IHC, a concentration of 3 ug/mL is recommended. Biosensis recommends optimal dilutions/concentrations should be determined by the end user. |
| Specificity |
Specificity has been confirmed by WB and direct ELISA against the antigen. |
| Cross-reactivity |
Human. Other species have not been tested. |
| Blast it |
To see the shared identity between different species or other proteins, follow the link in the Accession field, select the sequence that you are interested in and copy and paste it HERE and blast/format it. |
| Form |
Lyophilised from PBS pH 7.2 |
| Reconstitution |
Reconstitute in 100 uL of sterile water. Centrifuge to remove any insoluble material. |
| Storage |
After reconstitution keep aliquots at -20C for higher stability or at 2-8C with an appropriate antibacterial agent. Glycerol (1:1) may be added for additional stability. Avoid repetitive freeze/thaw cycles. |
| Expiry Date |
12 months after purchase |
