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Mouse monoclonal antibody to human muscarinic acetylcholine receptor [4B5]: IgG

$327.00USD


Catalogue No. M-814-100
Description The muscarinic acetylcholine receptor belongs to the G-protein coupled receptor 1 family and mediates various cellular responses such as adenylate cyclase inhibition, phosphoinositide breakdown and potassium channel modulation through the action of G proteins. The unprocessed precursor has a length of 466 amino acids and a predicted molecular weight of 51.7KDa.
Batch No. See product label
Unit size 100 ug
Antigen Partial recombinant protein of human muscarinic acetylcholine receptor (amino acids 251 to 350) with a GST tag.
Isotype IgG2a Kappa
Clone 4B5
Other Names CHRM2
Accession ACM2_HUMAN
Produced in Mouse
Purity Protein G purified immunoglobulin
Applications This antibody is recommended for WB, direct and sandwich ELISA. The detection limit of recombinant GST tagged muscarinic acetylcholine receptor is 0.03ng/mL when using mouse monoclonal antibody to recombinant GST muscarinic acetylcholine receptor [4B5], catalogue number M-814-100, as a capture antibody. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Specificity The reactivity of this antibody has been confirmed by WB and ELISA against the recombinant GST tagged muscarinic acetylcholine receptor protein.
Cross-reactivity This antibody has not yet been tested against other species.
Blast it If you would like to see the shared identity between different species or other proteins, follow the link in the Accession field, select the sequence (make sure that you are selecting the sequence that you are interested in, as the sequence may be the precursor rather than the mature protein for example) and copy and paste it HERE and blast/format it.
Form Lyophilised from PBS pH 7.4
Reconstitution Reconstitute in 100 uL of sterile water. Centrifuge to remove any insoluble material.
Storage Store lyophilized antibody at 2-8C. After reconstitution keep aliquots at -20C for higher stability or at 2-8C with an appropriate antibacterial agent. Glycerol (1:1) may be added for additional stability. Avoid repetitive freeze/thaw cycles.
Expiry Date 12 months after purchase
Images (click to zoom)
Mouse monoclonal antibody to human muscarinic acetylcholine receptor [4B5]: IgG Western blot analysis on GST tagged recombinant ACM2 protein using mouse monoclonal antibody to human muscarinic acetylcholine receptor [4B5], catalogue number M-814-100. Note the GST tag alone has a molecular weight of 26KDa.

PDF Data Sheet

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MSDS

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