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Mouse monoclonal antibody to human PIP [2E1]: IgG

$327.00USD


Catalogue No. M-805-100
Description PIP belongs to the inositol monophosphatase family. It hydrolyses adenosine 3'phosphate 5'-phosphosulfate to adenosine 5-phosphosulfate and 3'(2)'-phosphoadenosine 5'-phospate to AMP. It requires magnesium as a cofactor. Lithium is an uncompetive inhibitor and inositol 1,4-bisphosphate is a competitive inhibitor of PIP. Lithium is one of the drugs used to treat manic depression and the inhibition of PIP may account for lithium-induced nephrotoxicity.
Batch No. See product label
Unit size 100 ug
Antigen Recombinant protein of amino acids 1 to 100 of human PIP with a GST tag.
Clone [2E1]
Other Names 3' (2'); 5'-bisphosphate nucleotidase 1; bisophosphotase 3'-nucleotidase 1; PAP-inositol-1;4-phosphotase; BPNT1
Accession BPNT1_HUMAN
Produced in Mouse
Purity Protein G purified immunoglobulin
Applications This antibody is recommended for WB, ELISA, sandwich ELISA, IHC and immunofluorescence. It is recommended to be used at 3ug/mL for IHC and 10ug/mL for immunofluorescence. This antibody has a detection limit of 0.03ng/mL in sandwich ELISA when using the recombinant protein. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Specificity The specificity of this antibody has been confirmed by WB against the recombinant protein and in HeLa cell lysate.
Cross-reactivity This antibody has not yet been tested in other species.
Blast it If you would like to see the shared identity between different species or other proteins, follow the link in the Accession field, select the sequence (make sure that you are selecting the sequence that you are interested in, as the sequence may be the precursor rather than the mature protein for example) and copy and paste it HERE and blast/format it.
Form Lyophilised from PBS pH 7.2
Reconstitution Reconstitute in 100 uL of sterile water. Centrifuge to remove any insoluble material.
Storage After reconstitution keep aliquots at -20C for higher stability or at 2-8C with an appropriate antibacterial agent. Glycerol (1:1) may be added for additional stability. Avoid repetitive freeze/thaw cycles.
Expiry Date 12 months after purchase
Images (click to zoom)
Mouse monoclonal antibody to human PIP [2E1]: IgG Immunohistochemistry of mouse monoclonal antibody to human PIP [2E1], catalogue number M-805-100, at a concentration of 3µg/ml, on formalin-fixed and paraffin-embedded human pancreas.
Mouse monoclonal antibody to human PIP [2E1]: IgG Immunofluorescence detection of PIP on HeLa cells when using mouse monoclonal antibody to human PIP [2E1], catalogue number M-805-100, at a concentration of 10g/ml.
Mouse monoclonal antibody to human PIP [2E1]: IgG Western blot analysis of PIP in HeLa cell lysate using the mouse monoclonal antibody to human PIP [2E1], catalogue number M-085-100. This anitbody recognises a band of approximate molecular weight 37 KDa
PDF Data Sheet

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MSDS

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