biosensis fine bioscience tools - antibodies, elisa kits, proteins, toxins and peptides An Australia-US partnershipPhone: 1800 605 5127 in the US, or +61 8 8352 7711 for international enquiries to Australia.
HomeAbout BiosensisBiosensis distributorsResourcesHelp deskordering from BiosensisContact usFlyers

Technical Notes
View our tech notes
Follow us...
Google+ Follow us on Twitter

Find us on Facebook

Sign up for Biosensis newsletters
Visit our newsletter archive

Mouse monoclonal antibody to human p75NTR [ME20.4] - ATTO 488


Catalogue No. M-027-50-AT
Description Nerve growth factor receptor (NGFR) is also referred to as p75(NTR) due to its molecular mass and its ability to bind at low affinity not only NGF (see 162030), but also other neurotrophins, including brain-derived neurotrophic factor (BDNF; 113505), neurotrophin-3 (NTF3; 162660), and neurotrophin-4/5 (NTF5; 162662). At the time of its discovery, NGFR was considered a unique type of protein. Subsequently, however, a large superfamily of tumor necrosis factor receptors were found to share the overall structure of NGFR (4 extracellular ligand-binding, cysteine-rich repeats, or CRs, and signaling through association with, or disassociation from, cytoplasmic interactors). The identification of this superfamily helped elucidate some of the biologic functions of NGFR, including its ultimate involvement in the nuclear factor kappa-B (NFKB; see 164011) and apoptosis pathways. As a monomer, NGFR binds NGF with low affinity. Higher affinity binding is achieved by association with higher molecular mass, low-affinity neurotrophin receptors, namely the tropomyosin receptor kinases, TRKA (NTRK1; 191315), TRKB (NTRK2; 600456), and TRKC (NTRK3; 191316). TRKA, TRKB, and TRKC are specific for or 'preferred by' NGF, NTF5 and BDNF, and NTF3, respectively (Ip et al., 1993). NTF3 also binds to TRKA and TRKB, but with significantly lower affinity
Batch No. See product label
Unit size 50 g
Antigen The p75NTR antibody was derived from immunization of mice with human WM245 melanoma cells.
Clone ME20.4
Other Names Low-affinity nerve growth factor receptor; NGF receptor; Gp80-LNGFR; p75 ICD; Low affinity neurotrophin receptor p75NTR
Accession TNR16_HUMAN
Produced in Mouse
Purity Protein G purified IgG was labelled with ATTO 488 and free dye removed by gel filtration.
Applications This antibody is recommended for use in immunohistochemistry, immunofluorescence, flow cytometry and NGF receptor p75 dynamics. For immunohistochemistry a concentration of 2 g/mL is recommended. Not appropriate for Western Blots. For FACS a concentration of 20 g/mL is recommended and for 1 site ELISA at least a 1 in 5000 dilution. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Specificity This antibody recognises p75NTR (low affinity neurotrophin receptor)
Cross-reactivity Reacts with human, cat, dog, pig, rabbit and sheep. Does not react with rat or mouse.
Form Liquid (1 mg/mL) in PBS, pH 7.2-7.6 without preservatives.
Storage Aliquot antibody and store frozen at -20C to -80C. For short-term storage, the antibody conjugate can be stored at 2-8C for up to 4 months with the addition of appropriate antibacterial agent, or for up to 1 week without the addition of a preservative.
Expiry Date Four months after purchase
References 1.Walker BR et al. (2007) Behav Brain Res 176:109-20
2. Ross A.H. et al. (1984) Characterization of nerve growth factor receptor in neural crest tumors using monoclonal antibodies. Proc Natl Acad Sci U S A. 1984 Nov;81(21):6681-5. ORIGINAL PAPER
Images (click to zoom)
Mouse monoclonal antibody to human p75NTR [ME20.4] - ATTO 488 Photostability of ATTO 488 compared to common dyes in water. Excitation at 488nm with 1W Argon-Ion Laser.

PDF Data Sheet

Click to download the data sheet - Click to download the Data Sheet


Click to download the MSDS - Click to download the MSDS

Offices in the USA and Australia
My Ice Bucket  
0 items
Email This Page
Tell someone you know about this product.
We Accept PayPal
You can use secured services of paypal to send us money via our email account MODULE_PAYMENT_PAYPAL_IPN_IDYou can use secured services of paypal to send us money via our email account MODULE_PAYMENT_PAYPAL_IPN_IDYou can use secured services of paypal to send us money via our email account MODULE_PAYMENT_PAYPAL_IPN_ID
Click to make a payment

Official PayPal Seal