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Mouse monoclonal antibody to human p75NTR [MLR2] - FITC


Catalogue No. M-018-100-FT
This product is no longer available. Please see below for our recommended alternative products.
Replaced by M-1819-50, Mouse monoclonal antibody to human NGFR/p75NTR [8J2] - FITC
Related products M-1763-100-FT, Non-specific Control IgG, clone X63 (Monoclonal) - FITC
Batch No. See product label
Unit size 100 µg
Antigen Human recombinant p75
Isotype IgG2a
Clone MLR2
Other Names Low-affinity nerve growth factor receptor; NGF receptor; Gp80-LNGFR; p75 ICD; Low affinity neurotrophin receptor p75NTR; Tumor necrosis factor receptor superfamily member 16; CD271; NGFR; TNFRSF16;
Accession P08138 TNR16_HUMAN;
Produced in Mouse
Purity Immunoglobulin (IgG2A) was purified using Protein G column (Amersham Pharmacia), polished with Sephacryl 200HR (Amersham Pharmacia) in PBS. The antibody was then conjugated to Fluorescein isomer 1 (FITC, Sigma). A minimum fluorescein: protein ratio of 3:1 is guaranteed. The conjugate was purified via gel filtration using a G25 fine grain gel in 10 mMTris/50mM NaCl solution.
Applications Immunofluorescence, flow cytometry. Suggested working dilutions: For immunofluorescence a concentration of 1-5 µg/ml is recommended. LIGHT fixation is a must, or unfixed works best. Epitope is sensitive to fixation. This antibody is not recommended for denaturing WB applications. For FACS a concentration of 20 µg/ml is recommended. This antibody does not block Neurotrophin binding. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Specificity Specificity was demonstrated by immunohistochemistry and FACS analysis. Immunohistochemical staining of motor neurons in spinal cord following lesion of sciatic nerve and in cholinergic neurons of the basal forebrain corresponding to published literature.
Cross-reactivity Reacts with human, mouse and rat. Other species have not yet been tested but it is expected that this antibody will be useful for the study of p75 in primates and other species.
Form Liquid. Buffer is 10 mM disodium phosphate, 1.75 mM monopotassium phosphate, 2.7 mM potassium chloride, 135 mM sodium chloride (PBS), pH 7.4. Contains no preservatives.
Storage The antibody conjugate can be stored at 4şC for up to 4 months with the addition of appropriate antibacterial agent.
Expiry Date Four months after purchase
Specific References Han YC et al. (2016) Direct Reprogramming of Mouse Fibroblasts to Neural Stem Cells by Small Molecules. Stem Cells Int. 2016;2016:4304916.

Han YC & Zhou XF. (2015) Method of producing multipotent stem cells. US 20150322405 A1 (patent).

Chacón PJ, Arévalo MA, Tébar AR. (2010) NGF-activated protein tyrosine phosphatase 1B mediates the phosphorylation and degradation of I-kappa-Balpha coupled to NF-kappa-B activation, thereby controlling dendrite morphology. Mol Cell Neurosci. 2010 Apr;43(4):384-93.

Wiese S, Herrmann T, Drepper C, Jablonka S, Funk N, Klausmeyer A, Rogers ML, Rush RA & Sendtner M (2009) Isolation and enrichment of embryonic mouse motoneurons from the lumbar spinal cord of individual mouse embryos. Nat Protoc. 2010;5(1):31-8.
References 1. Rogers ML, Atmosukarto I, Berhanu DA, Matusica D, Macardle P, Rush RA (2006). Functional monoclonal antibodies to p75 neurotrophin receptor raised in knockout mice. Neurosci Methods. 158(1) pp. 109-120
2. Matusica D, Fenech MP, Rogers ML, Rush RA. (2008) Characterisation and use of the NSC-34 cell liner for study of nerurotrophin receptor trafficking. J. Neurosci. Res. 86(3) pp. 553-65.
3. Huh CY, Danik M, Manseau F, Trudeau LE, Williams S. (2008) Chronic exposure to nerve growth factor increases acetylcholine and glutamate release from cholinergic neurons of the rat medial septum and diagonal band of Boca via mechanisms mediated bu p75NTR. J. Neurosci. 28(6) pp. 1404-9.
4. Lagares A, Li HY, Zhou XF, Avendano C. (2007) Primary sensory neuron addition in the adult rat trigeminal ganglion: evidence for neural crest glio-neuronal precursor maturation. J. Neurosci. 27(30) pp. 7939-53.
Images (click to zoom)
Mouse monoclonal antibody to human p75NTR [MLR2] - FITC Live confocal imaging of intracellular trafficking of p75NTR using FITC labelled mouse monoclonal antibody to human p75NTR [MLR2], catalogue number M-018-100-FT in NSC34 mouse motor neuron cell line.

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