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Mouse monoclonal antibody to human Semaphorin-4D [3B4]: IgG

$327.00USD


Catalogue No. M-888-100
Description Semaphorin-4D is an 863-amino acid polypeptide containing a secretory signal sequence, a single transmembrane domain, an immunoglobulin (Ig)-like domain, and a conserved 500-amino acid sema domain. It was first identified as a cell surface protein of resting T cells and was the first semaphorin believed to be involved in the immune system. In addition to playing a functional role in the immune system it may also act in the nervous system. It induces B-cells to aggregate and improves their viability in vitro. It is strongly expressed in skeletal muscle, peripheral blood lymphocytes, spleen and thymus. It is expressed at lower levels in testes, brain, kidney, small intestine, prostate, heart, placenta, lung and pancreas, but not in colon or liver.
Batch No. See product label
Unit size 100 ug
Antigen Partial recombinant human Semaphorin-4D (115-224) with a GST tag.
Clone 3B4
Other Names BB18; A8; GR3; CD100 antigen; SEMA4D; CD100; SEMAJ
Accession SEM4D_HUMAN
Produced in Mouse
Purity Protein G purified immunoglobulin
Applications This antibody is recommended for WB, and sandwich ELISA. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Specificity Specificity has been confirmed by WB and direct ELISA against the antigen.
Cross-reactivity Human. Other species have not been tested.
Blast it To see the shared identity between different species or other proteins, follow the link in the Accession field, select the sequence that you are interested in and copy and paste it HERE and blast/format it.
Form Lyophilised from PBS pH 7.2
Reconstitution Reconstitute in 100 uL of sterile water. Centrifuge to remove any insoluble material.
Storage After reconstitution keep aliquots at -20C for higher stability or at 2-8C with an appropriate antibacterial agent. Glycerol (1:1) may be added for additional stability. Avoid repetitive freeze/thaw cycles.
Expiry Date 12 months after purchase
Images (click to zoom)
Mouse monoclonal antibody to human Semaphorin-4D [3B4]: IgG Western blot detection of human Semaphorin-4D expression in human promyelocytic leukemia whole cell lysate.
Mouse monoclonal antibody to human Semaphorin-4D [3B4]: IgG Sandwich ELISA using the anti-Semaphorin-4D monoclonal antibody as a capture antibody. The detection limit for GST tagged recombinant Semaphorin-4D is 0.3 ng/ml.
PDF Data Sheet

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MSDS

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