||Tyrosine kinase receptor type B (TrkB) is a member of the neurotrophic tyrosine receptor kinase family. It is a membrane-bound receptor and upon neurotrophin binding, it phosphorylates itself as well as MAPK pathways members. TrkB is the receptor for brain-derived neurotrophic factor (BDNF), neurotrophin-3 and neurotrophin-4/5, but not nerve growth factor (NGF). It is involved in the development and/or maintenance of the nervous system. It exists in a dynamic equilibrium between monomeric (low affinity) and dimeric (high affinity) structures. It is a tran-membrane protein. There are 3 named isoforms produced by alternative splicing. Additional isoforms seem to exist. The different forms are differentially expressed in various cell types including neurons and microglia.
||See product label
||Recombinant protein of human Tyrosine kinase receptor type B (isoform TrkB-T1) with a GST tag.
||Tropomyosin-related kinase receptor; BDNF/NT-3 growth factors receptor; Neurotrophic tyrosine kinase receptor type 2; TrkB tyrosine kinase; GP145-TrkB; Trk-B; TrkB; NTRK2; TRKB
||Protein G purified immunoglobulin
||This antibody is recommended for WB, sandwich ELISA and Flow Cytometry. Use 2 ug/10^6 cells for Flow Cytometry. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
||Specificity has been confirmed by WB and direct ELISA against the antigen.
||Human. Other species have not been tested.
||To see the shared identity between different species or other proteins, follow the link in the Accession field, select the sequence that you are interested in and copy and paste it HERE and blast/format it.
||Lyophilised from PBS pH 7.2
||Reconstitute in 100 uL of sterile water. Centrifuge to remove any insoluble material.
||After reconstitution keep aliquots at -20C for higher stability or at 2-8C with an appropriate antibacterial agent. Glycerol (1:1) may be added for additional stability. Avoid repetitive freeze/thaw cycles.
||12 months after purchase
||1. Mizoguchi Y et al., J Immunol. 2009 Dec 15;183(12):7778-86.
2. Spencer-Segal JL et al. J Neurosci. 2011 May 4;31(18):6780-90.
3. Nakajima K et al. Glia. 1998 Nov;24(3):272-89.