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Mouse monoclonal antibody to the human Tyrosine kinase receptor type B [4D3-F10]: IgG


Catalogue No. M-853-100
Description Tyrosine kinase receptor type B (TrkB) is a member of the neurotrophic tyrosine receptor kinase family. It is a membrane-bound receptor and upon neurotrophin binding, it phosphorylates itself as well as MAPK pathways members. TrkB is the receptor for brain-derived neurotrophic factor (BDNF), neurotrophin-3 and neurotrophin-4/5, but not nerve growth factor (NGF). It is involved in the development and/or maintenance of the nervous system. It exists in a dynamic equilibrium between monomeric (low affinity) and dimeric (high affinity) structures. It is a tran-membrane protein. There are 3 named isoforms produced by alternative splicing. Additional isoforms seem to exist. The different forms are differentially expressed in various cell types including neurons and microglia.
Batch No. See product label
Unit size 100 ug
Antigen Recombinant protein of human Tyrosine kinase receptor type B (isoform TrkB-T1) with a GST tag.
Isotype IgG2a, kappa
Clone 4D3-F10
Other Names Tropomyosin-related kinase receptor; BDNF/NT-3 growth factors receptor; Neurotrophic tyrosine kinase receptor type 2; TrkB tyrosine kinase; GP145-TrkB; Trk-B; TrkB; NTRK2; TRKB
Accession NTRK2_HUMAN
Produced in Mouse
Purity Protein G purified immunoglobulin
Applications This antibody is recommended for WB, sandwich ELISA and Flow Cytometry. Use 2 ug/10^6 cells for Flow Cytometry. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Specificity Specificity has been confirmed by WB and direct ELISA against the antigen.
Cross-reactivity Human. Other species have not been tested.
Blast it To see the shared identity between different species or other proteins, follow the link in the Accession field, select the sequence that you are interested in and copy and paste it HERE and blast/format it.
Form Lyophilised from PBS pH 7.2
Reconstitution Reconstitute in 100 uL of sterile water. Centrifuge to remove any insoluble material.
Storage After reconstitution keep aliquots at -20C for higher stability or at 2-8C with an appropriate antibacterial agent. Glycerol (1:1) may be added for additional stability. Avoid repetitive freeze/thaw cycles.
Expiry Date 12 months after purchase
General References 1. Mizoguchi Y et al., J Immunol. 2009 Dec 15;183(12):7778-86.
2. Spencer-Segal JL et al. J Neurosci. 2011 May 4;31(18):6780-90.
3. Nakajima K et al. Glia. 1998 Nov;24(3):272-89.
Images (click to zoom)
Mouse monoclonal antibody to the human Tyrosine kinase receptor type B [4D3-F10]: IgG Left: Western blot detection of Tyrosine kinase receptor type B (TrkB) expression in human osterogenic sarcoma cell lysate. Right: Analysis of TrkB expression in human prostate cancer DU145 cell line  by Flow Cytometry. DU145 cells washed twice using PBS, Blocking: 200 ug/mL Normal Sheep IgG (20 minutes), Primary antibody: Mouse Monoclonal antibody to TrkB (cat # M-853-100, 2 μg per ~10^6 cells) for 30 minutes on ice, Secondary antibody: Goat anti-mouse PE labeled secondary antibody (1:100 fold dilution) with incubation for 20 minutes in dark at room temperature. Non-specific Control IgG, clone X63 (cat # M-1249-100) was used as negative control under same conditions (black dashed). Flow cytometry data and results were generated using Orflo MoxiflowTM instrument and protocols.

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