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Mouse monoclonal antibody to CD4 [CA-4]: IgG


Catalogue No. M-1165-100
Description CD4 is a single-pass type I membrane protein expressed on the surface of T helper/inducer cells, regulatory T cells, monocytes, macrophages, dendritic cells and microglia.
Batch No. See product label
Unit size 100 ug
Antigen CD4 transfected mouse T-cell hybridoma 3DT followed by CD4+ human T-cell CEM cell line
Clone CA-4
Other Names T-cell surface glycoprotein CD4; T-cell surface antigen T4/Leu-3; W3/25 antigen; CD4mut;
Accession P01730 CD4_HUMAN; P05540 CD4_RAT;
Produced in Mouse
Purity IgG
Applications Immunohistochemistry (IHC-Frozen). A concentration of 1.0 ug/mL is recommended to detect CD4 in formalin/acetone fixed tissues. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Specificity The specificity of this antibody has been confirmed by immunohistochemistry against the antigen.
Cross-reactivity Human; rat;
Blast it If you wish to use this product in a species other than those specified here and want to determine the amino acid sequence homology between the immunogen and the corresponding protein in that species, simply copy the amino acid sequence for this immunogen and test it on the BLAST database that you can access through the link below. Please note that antibodies raised against synthetic peptides are quite often very specific for that peptide. What this means in practice is that a single amino acid difference may be enough to restrict the specificity to a particular molecule. The BLAST database can also be used to compare any amino acid sequence between species and between protein. The full amino acid sequence of the target protein can be found by following the link in the Accession field. You can then search any amino acid sequence in the target protein using the BLAST database. When selecting a sequence to search, please choose carefully, as the sequence you choose may be a precursor rather than the mature protein for example.
Form Liquid (0.5ml). 50% glycerol, 0.9mg NaCl and 0.2mg Na2HPO4
Reconstitution The liquid formulation should be diluted in PBS (pH 7.4)
Storage After reconstitution, aliquot and store at -20C for a higher stability. Avoid freeze-thaw cycles.
Expiry Date 12 months after purchase
References 1. Almolda B. et al. CD4 microglial expression correlates with spontaneous clinical improvement in the acute Lewis rat EAE model. J Neuroimmunol. 2009 Apr 30;209(1-2):65-80.
2. Jordan C.A. et al. Infection of brain microglial cells by human immunodeficiency virus type 1 is CD4 dependent. J Virol. 1991 Feb;65(2):736-42.
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