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Mouse monoclonal antibody to DJ-1 [PARK7]: IgG


Catalogue No. M-1573-100
Description Protein DJ-1 has many roles including protecting cells against oxidative stress and cell death (Ref: SwissProt). Mutations in the DJ-1 gene have been associated with rare forms of autosomal recessive early-onset Parkinson's disease.
Batch No. See product label
Unit size 100 ug
Antigen Full length recombinant human DJ-1 expressed in and purified from E. coli.
Antibody Type monoclonal
Isotype IgG1/kappa
Clone 4H4
Other Names Oncogene DJ1; Parkinson disease protein 7; PARK7; DJ-1
Accession Q99497 PARK7_HUMAN;
Produced in Mouse
Applications WB, ICC. Suggested dilution of at least 1:500 for ICC. Dilutions of 1:5,000 or lower is recommended for WB. This antibody reveals a prominent ~21 kDa band and stains mainly in cytoplasm of tissue culture cells. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Specificity The antibody reacts with a 21 kDa band by Western blot on whole HeLa cell lysate. It has also been used successfully for immunocytochemistry.
Species Against Human.
Antibody Against DJ1 [PARK7]
Cross-reactivity Does not react with rat and mouse DJ-1 protein on western blots.
Blast URL Click here
Form Lyophilized from PBS. Contains 5% trehalose.
Appearance White powder
Reconstitution Reconstitute in sterile distilled water. Centrifuge to remove any insoluble material.
Storage After reconstitution of lyophilised antibody, aliquot and store at -20C for a higher stability. Avoid freeze-thaw cycles.
Expiry Date 12 months after purchase
General References 1. Nagakubo D, Taira T, Kitaura H, Ikeda M, Tamai K, Iguchi-Ariga SM and Ariga H DJ-1, a novel oncogene which transforms mouse NIH3T3 cells in cooperation with ras. Biochem. Biophys. Res. Commun. 231, 509–513 (1997).
2. Bonifati V, Rizzu P, van Baren, M.J., Schaap O, Breedveld GJ, Krieger E, Dekker MC, Squitieri F, Ibanez P, Joosse M et al. Mutations in the DJ-1 gene associated with autosomal recessive early-onset Parkinsonism. Science, 299, 256–259 (2003).
3. Xu J, Zhong N, Wang H, Elias JE, Kim CY, Woldman I, Pifl C, Gygi SP, Geula C, Yankner BA. The Parkinson's disease-associated DJ-1 protein is a transcriptional co-activator that protects against neuronal apoptosis. Hum Mol Genet. 14 (9):1231-41 (2005).
4. Yokota T, Sugawara K, Ito K, Takahashi R, Ariga H. And Mizusawa, H. Down regulation of DJ-1 enhances cell death by oxidative stress, ER stress, and proteasome inhibition. Biochem. Biophys. Res. Commun., 312, 1342–1348 (2003).
5. Taira T, Saito Y, Niki T, Iguchi-Ariga SM, Takahashi K and Ariga H. DJ-1 has a role in antioxidative stress to prevent cell death. EMBO Rep., 5, 213–218 (2004).
6. Bonifati V, Oostra BA. and Heutink, P. Linking DJ-1 to neurodegeneration offers novel insights for understanding the pathogenesis of Parkinson’s disease. J.Mol,Med.82,163-174 (2004).
Images (click to zoom)
Mouse monoclonal antibody to DJ-1 [PARK7]: IgG HeLa cells stained with Mouse monoclonal antibody to DJ-1 M-1573-100 (green), and Chicken polyclonal antibody to Vimentin C-1409-50 (red) and DNA (blue). M-1573-100 antibody reveals strong cytoplasmic staining for DJ-1.
Mouse monoclonal antibody to DJ-1 [PARK7]: IgG Whole HeLa cell lysate stained with mouse anti-DJ1 antibody. The antibody recognizes the ~21 kDa protein.
Mouse monoclonal antibody to DJ-1 [PARK7]: IgG Western blot analysis of whole brain and cell lysates using mouse antibody to DJ1 (green, 1:5,000). [1] protein standard, [2] rat brain, [3] mouse brain, [4] NIH-3T3, [5] HeLa, [6] HEK293, and [7] C6 cells. The antibody detects DJ1 protein with apparent molecular weight of 21 kDa, but only in human cell lines, since it does not recognize the rat or mouse DJ1 protein. The blot was simultaneously probed with chicken antibody to vimentin (C-1409-50, red, 1:5,000), revealing a single band at about 50 kDa (lanes 2-7).
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