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Mouse monoclonal antibody to Protein S100-beta [SH-B1]: IgG


Catalogue No. M-1746-100
Description Protein S100 beta belongs to the S100 family of proteins containing 2 EF-hand calcium binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and are involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. Among brain cells, S100 beta is often used as a marker for astrocytes. The 2 subunits of S100 protein are the products of separate genes. The beta subunit is present in all S100 positive cells and tumours whereas the alpha subunit is detectable only in specific tissues such as heart, skeletal muscle and brain. The S100 protein is a homodimer composed of two alpha subunits (S100a) or two beta subunits (S100b) or as a heterodimer with one alpha and beta subunit (S100ab or S100ao). This antibody reacts specifically with the beta subunit.
Batch No. See product label
Unit size 100 ug
Antigen Bovine brain S-100b
Antibody Type Mouse monoclonal
Clone SH-B1
Other Names S100B; Calcium binding protein B1;
Accession P02638 S100B_BOVIN
Produced in Mouse
Molecular Weight The predicted length of bovine S100 beta subunit is 92 residues and the MW of the monomer is 11 kDa.
Purity Ascites
Applications Immunohistochemistry (IHC). A concentration of 1.0-2.0 ug/mL is recommended to detect S100 beta subunit in paraffin embedded tissues. Antigen retrieval by heat is recommended. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Specificity The specificity of this antibody has been confirmed by IHC against the antigen.
Species Against Bovine
Cross-reactivity Human, rabbit and rat. May cross-react with horse and monkey S100-beta based on amino acid sequence similarities.
Blast it If you wish to use this product in a species other than those specified here and want to determine the amino acid sequence homology between the immunogen and the corresponding protein in that species, simply copy the amino acid sequence for this immunogen and test it on the BLAST database that you can access through the link below. Please note that antibodies raised against synthetic peptides are quite often very specific for that peptide. What this means in practise is that a single amino acid difference may be enough to restrict the specificity to a particular molecule. The BLAST database can also be used to compare any amino acid sequence between species and between protein. The full amino acid sequence of the target protein can be found by following the link in the Accession field. You can then search any amino acid sequence in the target protein using the BLAST database. When selecting a sequence to search, please choose carefully, as the sequence you choose may be a precursor rather than the mature protein for example.
Form Lyophilized from mouse ascites fluid containing 1.2% sodium acetate, 2 mg BSA, with 0.01 mg NaN3 as preservative.
Reconstitution Reconstitute in 1 mL of PBS buffer to achieve an antibody concentration of 100 ug/mL. Centrifuge to remove any insoluble material.
Storage 12 months after purchase at -20C (lyophilized formulations). After reconstitution, aliquot and store at -20C for a higher stability. Avoid freeze-thaw cycles.
Expiry Date 12 months after purchase.
PDF Data Sheet

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