| Catalogue No. |
M-994-100 |
| Description |
Actinin is a F-actin cross linking protein and is thought to anchor actin to a variety of intracellular structures. At least 4 isoforms of alpha actinin have been found. This antibody reacts specifically with alpha actinin isoform #1. |
| Batch No. |
See product label |
| Unit size |
100 ug |
| Antigen |
Rabbit skeletal muscle alpha actinin |
| Clone |
SA-20 |
| Other Names |
ACTN1; |
| Accession |
P12814 ACTN1_HUMAN |
| Produced in |
Mouse |
| Purity |
IgG |
| Applications |
Immunohistochemistry (IHC) and Western Blotting (WB). A concentration of 3.5 ug/mL is recommended for WB. The predicted length of human actinin (isoform #1) is 892 residues and a MW of 103 kDa. A concentration of 3.5-7.0 ug/mL is recommended to detect alpha actinin in formalin fixed and paraffin embedded tissues. Antigen retrieval is required. Also the recommended titre for formalin/acetone fixed tissues. Biosensis recommends optimal dilutions/concentrations should be determined by the end user. |
| Specificity |
The specificity of this antibody has been confirmed by WB and IHC against the antigen. |
| Cross-reactivity |
Human; mouse; rat; |
| Blast it |
If you wish to use this product in a species other than those specified here and want to determine the amino acid sequence homology between the immunogen and the corresponding protein in that species, simply copy the amino acid sequence for this immunogen and test it on the BLAST database that you can access through the link below. Please note that antibodies raised against synthetic peptides are quite often very specific for that peptide. What this means in practise is that a single amino acid difference may be enough to restrict the specificity to a particular molecule.
The BLAST database can also be used to compare any amino acid sequence between species and between protein. The full amino acid sequence of the target protein can be found by following the link in the Accession field. You can then search any amino acid sequence in the target protein using the BLAST database. When selecting a sequence to search, please choose carefully, as the sequence you choose may be a precursor rather than the mature protein for example. |
| Form |
Lyophilized from 1.2% sodium acetate, 2mg BSA, 0.01mg NaN3 |
| Reconstitution |
Reconstitute in 1 mL of PBS (pH 7.4) to achieve an antibody concentration of 100 ug/mL. Centrifuge to remove any insoluble material. |
| Storage |
At least 12 months after purchase at 2-8C (lyophilized formulations). After reconstitution, aliquot and store at -20C for a higher stability. Avoid freeze-thaw cycles. |
| Expiry Date |
12 months after purchase. |
