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Mouse monoclonal antibody to Tyrosine Kinase Receptor C (TrkC, 397-408), extracellular domain [BS337]: IgG

$297.00USD


Catalogue No. M-1837-100
Description Receptor tyrosine kinase involved in nervous system and probably heart development. Upon binding of its ligand NTF3/neurotrophin-3, NTRK3 autophosphorylates and activates different signaling pathways, including the phosphatidylinositol 3-kinase/AKT and the MAPK pathways, that control cell survival and differentiation (Ref: uniprot.org).
Related products R-1835-100, Rabbit antibody to Tyrosine Kinase Receptor C (TrkC; 172-186): affinity purified
Batch No. See product label
Unit size 100 ug
Antigen Synthetic peptide immunogen, KEPFPESTDNFI
Sequence KEPFPESTDNFI
Antigen Location Extracellular domain of TrkC (amino acids 397-408 of human TrkC)
Antibody Type Mouse monoclonal
Isotype IgG2b, kappa
Clone BS337
Other Names GP145-TrkC; Trk-C; Neurotrophic tyrosine kinase receptor type 3; TrkC tyrosine kinase
Accession NTRK3_HUMAN
Produced in Mouse
Purity Protein G purified
Applications Flow Cytometry (5-10 ug/mL): Tested on human and rodent cell lines. Cell staining can be performed under native conditions on ice, or on fixed cells with up to 4% formaldehyde.
Immunocytochemistry (1-2 ug/mL): Tested on fixed (4% formaldehyde) human cells.
Other applications have not been tested. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Specificity TrkC
Species Against Human
Cross-reactivity Mouse and rat TrkC. Species cross-reactivity determined by Flow Cytometry.
Form Lyophilized from PBS, pH 7.2-7.6 with 0.1% trehalose.
Reconstitution Spin vial briefly before opening. Reconstitute in 100 uL sterile distilled water to obtain an antibody concentration of 1 mg/mL. Centrifuge to remove any insoluble material.
Storage Store lyophilised antibody at 2-8C. After reconstitution divide into aliquots and store at -20C for long-term storage. Store at 2-8C short-term (up to 4 weeks). Avoid repetitive freeze/thaw cycles.
Expiry Date 12 months after purchase if unopened
Images (click to zoom)
Mouse monoclonal antibody to Tyrosine Kinase Receptor C (TrkC, 397-408), extracellular domain [BS337]: IgG TrkC expression (red) in human (HeLa, SHSY5Y, MCF-7) and rat x mouse hybrid cell line 50B11 analysed by Flow Cytometry. Staining was performed on unfixed cells on ice. Blocking: 100 ug/mL normal sheep IgG in PBS (30 minutes) on ice. Primary antibody: TrkB clone BS337 (10 ug/mL) for 60 minutes on ice. Secondary antibody: Goat anti-rabbit PE (1:100 dilution), 30 min in dark on ice. Non-specific control IgG clone X63 (black) was used as negative control under same conditions. Flow cytometry data and results were generated using Orflo MoxiflowTM instrument and protocols.
Mouse monoclonal antibody to Tyrosine Kinase Receptor C (TrkC, 397-408), extracellular domain [BS337]: IgG TrkC expression (red) in 50B11 mouse x rat hybrid cell line analysed by Flow Cytometry. Fixation: 0 ,2 and 4% formaldehyde in PBS, pH 7.2-7.6 on ice (10 minutes). Blocking: 100 ug/mL normal sheep IgG in PBS (30 minutes) on ice. Primary antibody: TrkC clone BS337 (10 ug/mL) for 60 minutes on ice. Secondary antibody: Goat anti-rabbit PE (1:100 dilution), 30 min in dark on ice. Non-specific control IgG clone X63 (black) was used as negative control under same conditions. Flow cytometry data and results were generated using Orflo MoxiflowTM instrument and protocols.
Mouse monoclonal antibody to Tyrosine Kinase Receptor C (TrkC, 397-408), extracellular domain [BS337]: IgG Membrane TrkC expression (green) in human HeLa cells, analyzed by Immunocytochemistry. Fixed (4% formaldehyde) and blocked (10% normal horse serum) HeLa cells were incubated with TrkC antibody M-1837-100 (2 ug/mL, green) for 1 hour. Cells were then permeabilized, blocked (10% horse serum, 0.1% Triton X-100) and incubated with rabbit NF-H antibody R-1389-50 (1:500, red) for 1 hour. Primary antibody binding was visualized with secondary donkey anti-mouse-CF488A and donkey anti-rabbit-CF568 antibodies (2 ug/mL, 1 hour incubation). Cell nuclei were stained with DAPI (blue). Negative control staining was performed under identical conditions with isotype control mouse antibody and rabbit whole serum from an non-immunized animal. Confocal imaging settings were the same for antibody and control staining. Magnification: 60x. Specific staining is observed for TrkC in the cell membrane, while the NF-H antibody reveals intracellular filaments.
Mouse monoclonal antibody to Tyrosine Kinase Receptor C (TrkC, 397-408), extracellular domain [BS337]: IgG 3D confocal imaging of membrane TrkC expression (green) in human HeLa cell. TrkC-IR is concentrated in the elongated protrusion away from the cell body, while intracellular NF-H expression (red) is observed throughout the cell. Blue: DAPI nuclear stain.
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