biosensis fine bioscience tools - antibodies, elisa kits, proteins, toxins and peptides An Australia-US partnershipPhone: 1800 605 5127 in the US, or +61 8 8352 7711 for international enquiries to Australia.
HomeAbout BiosensisBiosensis distributorsResourcesHelp deskordering from BiosensisContact usFlyers

Technical Notes
View our tech notes
Follow us...
Google+ Follow us on Twitter

Find us on Facebook

Sign up for Biosensis newsletters
Visit our newsletter archive

Mouse monoclonal antibody to Ubiquitin [Ubi-1]


Catalogue No. M-1404-100
Description Ubiquitin is a highly conserved 76 amino acid protein with an estimated molecular weight of 8.56 kDa which has a central role in regulated protein degradation. It is a protein modifier which can be covalently attached to target lysines either as a monomer or as a lysine-linked polymer. Several types of polymeric chains can be formed depending on the lysine used for the assembly. Attachment to proteins as a polymer leads to their degradation by the 26S proteosome; a complex, multicatalytic cytosolic and nuclear protease. Attachment to proteins as a monomer or as an alternatively linked polymer does not lead to proteasomal degradation and may be required for numerous functions, including maintenance of chromatic structure, regulation of gene expression, stress response, ribosome biogenesis and DNA repair. Ubiquitin is synthesized as a polyubiquitin precursor with exact head to tail repeats, the number of repeats of which differ between species and strains. In some species there is a final amino-acid after the last repeat, here in bovine a Cys. Some ubiquitin genes contain a single copy of ubiquitin fused to a ribosomal protein (either L40 or S27a).
Batch No. See product label
Unit size 100 uL
Antigen Raised against purified ubiquitin conjugated with glutaraldehyde to keyhole limpet hemocyanin.
Antibody Type Monoclonal
Isotype IgG1
Clone Ubi-1
Other Names RPS27A; UBA52; UBB; UBC; Polyubiquitin-B; Polyubiquitin-C;
Accession P0CG47 UBB_HUMAN;
Produced in Mouse
Applications Western Blotting (WB), Immunohistochemistry - paraffin embedded tissue (IH-P) and ELISA. Suggested dilution for WB is 1:500-1,000. This antibody can be used on mildly fixed histological sections of human brain for studies of Alzheimer's disease. This antibody also works on paraffin embedded material. It also recognises other ubiquinated inclusion bodies such as Lewy bodies of Parkinson's disease and the Pick bodies in Pick's disease in formalin fixed tissues. Suggested dilution for IH is 1:500. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Specificity The specificity of this antibody has been confirmed by WB. This antibody detects ~8.5 kDa Ubiquitin.
Antibody Against Ubiquitin
Cross-reactivity Hu, Bov, Chk, Drosophila, and C. elegans
Blast URL Click here
Form Lyophilised with 5% trehalose
Appearance White powder
Reconstitution Reconstitute in sterile distilled water. Centrifuge to remove any insoluble material.
Storage After reconstitution of lyophilised antibody, aliquot and store at -20C for a higher stability. Avoid freeze-thaw cycles.
Expiry Date 12 months after purchase
Specific References 1. Josephs K.A. et al (2006) Atypical progressive supranuclear palsy with corticospinal tract degeneration. J Neuropathol Exp Neurol. 2006 Apr;65(4):396-405.
2. Josephs K.A. et al (2007) Neuropathologic features of frontotemporal lobar degeneration with ubiquitin-positive inclusions with progranulin gene (PGRN) mutations. J Neuropathol Exp Neurol. 2007 Feb;66(2):142-51.
3. Rudzinski L.A. et al (2008) Early onset familial Alzheimer Disease with spastic paraparesis, dysarthria, and seizures and N135S mutation in PSEN1. Alzheimer Dis Assoc Disord. 2008 Jul-Sep;22(3):299-307.
4. Josephs K.A. et al (2009) Evaluation of subcortical pathology and clinical correlations in FTLD-U subtypes. Acta Neuropathol. 2009 Sep;118(3):349-58.
Images (click to zoom)
Mouse monoclonal antibody to Ubiquitin [Ubi-1] Mouse monoclonal antibody to Ubiquitin [Ubi-1] M-1404-100 staining of cerebral cortex of an Alzheimer patient. Neurofibrillary tangles and dystrophic neurites associated with senile plaques stain strongly with this antibody. In the center is a typical neurofibrillary tangle containing neuron.
Mouse monoclonal antibody to Ubiquitin [Ubi-1] Left: Analysis of ubiquitin expression (brown) in FFPE section of human cerebral cortex of an Alzheimer patient by Immunohistochemistry. Signal was generated with DAB. Counterstain: haemotoxylin (blue). A typical flame-shaped tangle is seen in a pyramidal neuron in the center and is surrounded by dystrophic neurites, also strongly ubiquitin-positive. Both are commonly seen in cortical and hippocampal Alzheimer brain sections and are typical for this disease, but are rare or absent in healthy brain. Right: Western blot analysis of HEK293 cell lysates for ubiquitin expression (green, 1:1,000). [1] protein standard, [2] cells maintained in normal medium, [3] cells treated with 10 uM of proteasome inhibitor lactacystin (Lc) for 16 hours. The smear detected above the 200 kDa standard represents accumulation of ubiquitinated proteins in proteasome inhibitor-Lc treated cells. The prominent band at 8 kDa corresponds to monoubiquitin. The same blot was probed with an antibody to HSP60 as loading control.
PDF Data Sheet

Click to download the data sheet - Click to download the Data Sheet


Click to download the MSDS - Click to download the MSDS

Offices in the USA and Australia
My Ice Bucket  
0 items
Email This Page
Tell someone you know about this product.
We Accept PayPal
You can use secured services of paypal to send us money via our email account MODULE_PAYMENT_PAYPAL_IPN_IDYou can use secured services of paypal to send us money via our email account MODULE_PAYMENT_PAYPAL_IPN_IDYou can use secured services of paypal to send us money via our email account MODULE_PAYMENT_PAYPAL_IPN_ID
Click to make a payment

Official PayPal Seal