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Phospho-Sure RTD Neuronal Cell & Soft tissue extraction buffer


Catalogue No. B-100-PS
Description Detergents typically present in cell lysis buffers are thought to disrupt organelles and compartments and increase the exposure of soluble phospho-proteins to phosphatases and proteases thereby resulting in uncontrolled dephosphorylation and proteolysis.

Phospho-SureRTD Neuronal extraction buffer is optimized for the extraction of phosphorylated proteins from neuronal and other soft tissues types. The buffer extracts the phosphoproteins in a native state without the use of harsh detergents or oxidizers, and it is specially formulated to help maintain phosphoproteins and protect them from degradation better than traditional detergent based extraction buffers.
Batch No. See product label
Unit size 100 mL
Other Names Phosphosure
Applications Please download the protocol below for detailed instructions on how to use Phospho-Sure in neuronal and other soft tissues.
Featured Articles Refer to the publication by Suneja et al (2006) where this buffer was shown to improve recovery of phospho-proteins such as ERK1/2-P and SAP/JNK-P in the dissection and homogenization of guinea pig brain tissue.
Comments Phospho-Sure™ RTD™ is designed as both an isolation and extraction buffer and for best results the intact tissue should be bathed in ice-cold Phospho-sure™ buffer during excision of the tissue. Once the desired tissue piece is isolated in Phospho-sure™ RTD™ it may be transferred to a small volume of ice-cold Phospho-Sure™ RTD™ for disruption.
Form Powder containing no preservatives. Preparation of Phospho-Sure™ RTD™ is very simple. Simply add sterile ddH2O directly to the powdered solution in the supplied bottle, swirl briefly to dissolve the chemicals and use. The material contains no preservatives so sterile technique should always be used.
Appearance White powder
Reconstitution Add 93 mL of sterile double distilled water and mix by gently swirling, final volume 100 mL at 20C.
Storage The dry, unopened container should be stored at room temperature in a dry or desiccated location protected from light. Do not store in the refrigerator unless material is in a dry, moisture free environment. Material is hydroscopic so once the seal is broken it should be hydrated and not resealed while dry. Once hydrated, the buffer can be stored at 2-8C for up to 3 months. Solution can be frozen but clumping may occur upon thawing and is not recommended.
Expiry Date Powdered format can be stored up to 12 months after purchase.
Specific References 1. Suneja SK, Mo Z, Potashner SJ. (2006) Phospho-CREB and other phospho-proteins: improved recovery from brain tissue. J Neurosci Methods. 2006 Jan 30;150(2):238-41.
2. Elvira Mass, Dagmar Wachten, Anna C. Aschenbrenner, André Voelzmann, Michael Hochemail (2014) Murine Creld1 Controls Cardiac Development through Activation of Calcineurin/NFATc1 Signaling, Developmental Cell Volume 28, Issue 6, p711–726
Buffer protocol Click to download the protocolB-100-PS_as_at_March2013.pdf
MSDS Click to download the Material Safety Data SheetMSDS_B-100_B-200_as_at_Apr2014.pdf
Images (click to zoom)
Phospho-Sure RTD Neuronal Cell & Soft tissue extraction buffer Western blots of Guinea pig brain stem inferior colliculi samples homogenized in Phospho-Sure RTD (B-100-PS) or Lysis buffer and kept on ice for up to 120 min. Aliquots of the homogenates (30µg) were run on SDS-PAGE and proteins transferred to blotting membrane. Blots were stripped and incubated with 3 antibodies: CREB-P, SAP/JNK-P and ERK 1/2. Image courtesy of Suneja et al (2006).
Phospho-Sure RTD Neuronal Cell & Soft tissue extraction buffer Figure shows the decrease in the decay rate in phosphorylated signals from Western Blots performed on samples held at RT for varying times in Phospho-Sure™ RTD™ and traditional lysis buffer preparations.
PDF Data Sheet

Click to download the data sheet - Click to download the Data Sheet

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