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Rabbit antibody to GFR alpha-3 (1-11): whole serum


Catalogue No. R-068-100
Description FUNCTION: Receptor for the glial cell line-derived neurotrophic factor, artemin. Mediates the artemin-induced autophosphorylation and activation of the RET receptor tyrosine kinase (By similarity). SUBCELLULAR LOCATION: Cell membrane; lipid-anchor; GPI-anchor (By similarity). SIMILARITY: Belongs to the GDNFR family.
Batch No. See product label
Unit size 100 uL
Antigen A synthetic peptide (MGLSWSPRPPL) as part of mouse GFR alpha-3 protein (aa: 1-11) conjugated to KLH
Other Names GDNF family receptor alpha-3; GFR-alpha-3
Accession GFRA3_MOUSE
Produced in Rabbit
Purity Whole serum
Applications IHC, WB. A dilution of 1:500 to 1:1000 is recommended for both applications. This antibody stains nicely large neurons in the rat DRG. Specifically, if you would like to perform WB using rat brain homogenate detecting GFR alpha-3, this is the antibody to be used. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Specificity Western blot analysis indicates a high level of specificity to GFR alpha-3 for this antiserum.
Cross-reactivity This antibody is known to react with rat GFR alpha-2.
Blast it If you would like to use this product in another species other than those specified here, or to see the shared ID between the immunogen used here in different speices and/or other molecules, simply copy the immunogen (from the Immunogen field) and paste it HERE and blast/format it. Note that, antisera raised against synthetic peptides are quite often very specific for that peptide ie, only one single amino acid difference may be enough to restrict the specificity to a particular molecule. Regardless, you can always contact us if you need assistance with this.
Form Lyophilised
Reconstitution Reconstitute in 100 uL of sterile water. Centrifuge to remove any insoluble material.
Storage After reconstitution keep aliquots at -20C for a higher stability, and at 2-8C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
References Naveilhan et al. (1998) Proc. Natl. Acad. Sci. USA. 95(3): 1295
Images (click to zoom)
Rabbit antibody to GFR alpha-3 (1-11): whole serum Immunohistochemical staining of rat dorsal root ganglia using Rabbit antibody to to GFR alpha-3 (1-11): whole serum, catalogue no. R-068-100.

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