| Catalogue No. |
R-145-100 |
| Description |
ATG10 plays a role in autophagy. It is an E2-like enzyme involved in 2 ubiquitin-like modifications essential for autophagosome formation: ATG12-ATG5 conjugations and modificatioon of a soluble form of MAPLC3 1A, a homolog of yeast ATG8, to a membrane bound form. It is also able to directly interact either with ATG5 or ATG7. |
| Batch No. |
See product label |
| Unit size |
100 uL |
| Antigen |
A peptide (CLKNSQKINKNV) corresponding to the C-terminal of human ATG10 protein has been used as the immunogen. |
| Other Names |
Autophagy-related protein 10; APG10-like; ATG10 |
| Accession |
ATG10_HUMAN |
| Produced in |
Rabbit |
| Purity |
Whole serum |
| Applications |
IHC, immunofluorescence. A dilution of 1:100 to 1:1000 dilution is recommended for these applications. Biosensis recommends optimal dilutions/concentrations should be determined by the end user. |
| Specificity |
IHC, WB and ELISA confirmed the specificity for ATG10. |
| Cross-reactivity |
Human. Other species not yet tested. |
| Form |
Lyophilised |
| Reconstitution |
Reconstitute in 100 uL of sterile water. Centrifuge to remove any insoluble material. |
| Storage |
After reconstitution keep aliquots at minus 20C for a higher stability, and at 2-8C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles. |
| Expiry Date |
Six months after purchase |
| Specific References |
1. Ryningen A et al (2012) Inhibition of Mammalian target of rapamycin in human acute myeloid leukemia cells has diverse effects that depend on the environmental in vitro stress.
Bone Marrow Res. 2012;2012:329061.
|
| References |
1. Wan D. et al. Proc. Natl. Acad. Sci. U.S.A. 101:15724-15729 (2004)
2. Ota T. et al. Nat. Genet. 36:40-45(2004)
3. The MGC Project Team. Genome Res. 14:2121-2127(2004). |
