| Catalogue No. |
R-084-100 |
| Description |
This product is no longer available. Please see below for our recommended alternative products. |
| Replaced by |
Mouse monoclonal antibody to rhBDNF, Clone 4C8, IgG, cat# M-1744-50/100
Rabbit polyclonal antibody to rhBDNF, affinity-purified, cat# R-1707-100
Rabbit polyclonal antibody to rhBDNF, whole serum, cat# R-088-100
Rabbit polyclonal antibody to rhBDNF, IgG, cat# R-017-500
Rabbit polyclonal antibody to BDNF (129-138), whole serum, cat# R-083-100
Rabbit polyclonal antibody to BDNF (129-138), IgG, cat# R-066-100 |
| Batch No. |
See product label |
| Unit size |
100 µl |
| Antigen |
A synthetic peptide (HSDPARRGELSVCDSISEWV) as a part of human BDNF protein (aa: 129-148) conjugated to KLH has been used as the immunogen. This sequence is highly conserved amongst mammals. |
| Other Names |
Brain-derived neurotrophic factor; Abrineurin; proBDNF; |
| Accession |
P23560 BDNF_HUMAN; |
| Produced in |
Rabbit |
| Purity |
Whole serum |
| Applications |
WB. A dilution of 1:500 to 1:2000 is recommended. For IHC applications, we recommend R-172-20. Biosensis recommends optimal dilutions/concentrations should be determined by the end user. |
| Specificity |
This antiserum specifically recognises recombinant BDNF and also detects a ~35 kDa band corresponding to the molecular weight of proBDNF in rat brain extract. No cross reactivity with NGF, NT3 or NT4/5 was detected by western blot. |
| Cross-reactivity |
This antiserum is known to react rat, human and mouse BDNF. |
| Blast it |
If you would like to use this product in another species other than those specified here, or to see the shared ID between the immunogen used here in different species and/or other molecules, simply copy the immunogen (from the Immunogen field) and paste it HERE and blast/format it. Note that, antisera raised against synthetic peptides are quite often very specific for that peptide ie, only one single amino acid difference may be enough to restrict the specificity to a particular molecule. Regardless, you can always contact us if you need assistance with this. |
| Form |
Lyophilised |
| Reconstitution |
Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material. |
| Storage |
After reconstitution keep aliquots at -20ºC for a higher stability, and at 4ºC with an appropriate antibacterial agent. Glycerol (1:) may be added for an additional stability. Avoid repetitive freeze/thaw cycles. |
| Expiry Date |
12 months after purchase |
| Specific References |
1. Puerta E. et al (2010) Sildenafil protects against 3-nitropropionic acid neurotoxicity through the modulation of calpain, CREB, and BDNF Neurobiol Dis. 2010 May;38(2):237-45.
|
| References |
BDNF Antibodies for Western Blotting |
