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Rabbit antibody to Caspase-3: Affinity Purified

$397.00USD


Catalogue No. R-1823-100
Description Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage.
Batch No. See product label.
Unit size 100 ug
Antigen E.coli-derived human Caspase 3 recombinant protein (Position: T67-D175) was used as the immunogen. Human Caspase 3 shares 86% and 90% amino acid (aa) sequences identity with mouse and rat Caspase 3, respectively.
Sequence 70 80 90 100 DVD AANLRETFRN LKYEVRNKND LTREEIVELM 110 120 130 140 150 RDVSKEDHSK RSSFVCVLLS HGEEGIIFGT NGPVDLKKIT NFFRGDRCRS 160 170 175 LTGKPKLFII QACRGTELDC GIETD
Antibody Type Polyclonal
Other Names CASP-3; Apopain; Cysteine protease CPP32; CPP-32; Protein Yama; SREBP cleavage activity 1; SCA-1; CASP3; CPP32
Accession P42574 CASP3_HUMAN
Produced in Rabbit
Purity Affinity purified
Applications Western Blot (0.1-0.5 ug/mL),: tested in human hepatocarcinoma cell line SMMC, rat liver tissue, rat thymus tissue Immunohistochemistry ? paraffin embedded section (0.5-1.0 ug/mL): tested in human lung cancer tissue, rat intestine tissue, mouse intestine tissue, Flow Cytometry (1-3 ug/10^6 cells): tested in human cell line K562. Other applications not yet tested. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Specificity This antibody is specific for caspase 3 as demonstrated by western blotting.
Species Against Human.
Antibody Against Caspase 3
Cross-reactivity Human, Mouse, Rat.
Form Lyophilized.
Reconstitution Spin vial briefly before opening. Reconstitute in 100 uL sterile, ultrapure water to prepare 1 mg/mL solution. Centrifuge to remove any insoluble material.
Storage Store lyophilised antibody at 2-8C. After reconstitution divide into aliquots and store at -20C for long-term storage. Store at 2-8C short-term (up to 4 weeks) with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles.
Expiry Date 12 months after purchase if unopened.
Images (click to zoom)
Rabbit antibody to Caspase-3: Affinity Purified A - Western blot analysis of Caspase 3 expression in rat liver (1), rat thymus (2) and SMCC whole cell lysate. Caspase-3 is detected at the expected molecular weight of 31 kDa. Samples were loaded at 50 ug/lane, and SDS-PAGE performed under reducing conditions. Transfer: nitrocellulose membrane. Blocking: 5% skim milk, 1.5 h at RT. Primary antibody: 0.5 ug/mL overnight at 2-8C. Secondary antibody: Goat anti-rabbit IgG-HRP (1:10,000), 1.5 h at RT. Detection: ECL.
B - Analysis of Caspase 3 expression in paraffin-embedded section of human lung cancer tissue by Immunohistochemistry. Heat-mediated antigen retrieval was performed in citrate buffer (pH6) for 20 minutes. The tissue section was blocked with 10% goat serum and then incubated with 1 ug/ml rabbit anti-caspase 3 antibody overnight at 2-8C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using DAB as the chromogen.
Rabbit antibody to Caspase-3: Affinity Purified C - Analysis of Caspase 3 expression in paraffin-embedded section of rat intestine tissue by Immunohistochemistry. Heat-mediated antigen retrieval was performed in citrate buffer (pH6) for 20 minutes. The tissue section was blocked with 10% goat serum and then incubated with 1 ug/ml rabbit anti-caspase 3 antibody overnight at 2-8C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using DAB as the chromogen. D - Flow Cytometry analysis of caspase 3 expression (blue) in K562 cells. The cells were blocked with 10% normal goat serum, then incubated with primary antibody (1 ug/10^6 cells) for 30 minutes at 20C. Secondary antibody: fluorescent-labelled goat anti-rabbit (5-10 ug/10^6 cells), 30 minutes at 20C. Isotype control antibody (green) and unlabelled sample (red) were used as control conditions.
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MSDS

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